Dynamic Change Of Neural Cell Adhesion Molecule L1 In Acute Spinal Cord Injury And Effect Of L1 Fusion Protein On Neural Axon Recovery

In the first part of experiment, we observed dynamic change of neural cell adhesion molecule L1 and some apoptosis index at different stage after spinal cord injury. Linearity positive correlation between L1/ β-actin and bcl-2/bax indicates that expression of L1 can inhibitor apoptosis. Expressive curve of nucleic acid and protein of L1 and GAP-43 was established in secondly part. The expression of GAP-43 insist on a short time; the level of mRNA for L1 maintain in high level for long time, which influence phosphorylated GAP-43 in growth cone. It may be notability in neurity outgrowth; The purpose of the third part was to investigate the neurite outgrowth of inthathecal infusion of exogenous fusion protein (His-Igl-6) on spinal cord injury using immunohistochemical staining, HRP of rubrospinal neurons and locomotor scale. It is authenticated that His-Igl-6 can reduce secondly injury of spinal cord and promote neurity recovery.