Research Of Insulin Resistant Related To Genome And Chinese Medical Pathogenesis And Treatment

ObjectiveTo study on the genome expression related with insulin resistance. Approach the pathogenesis of insulin resistance on the basis of Chinese medical science. Found the Chinese medical treatment to insulin resistance and illuminate its mechanism.Methods1. Set up the insulin resistance rat model with the feature of simple obesity by using high fat feedstuff. Observed rat's body weight, visceral fat weight and Lee's index. Detect FBG, FINS, FFA, TG, TC, HDL-C, LDL-C and blood rheology index by using biochemistry methods. Appraised IR by hyperinsulinemic-euglycemic clamp. Observed rat's histopathologic change of visceral adipose tissue, liver, kidney, pancreases, skeletal muscle and arch of aorta. Studied on the genome expression of adipose tissue around internal organs by gene chip technique.2. Set up the insulin resistance rat model with the feature of type 2 diabetes mellitus by using fat feedstuff and low dose STZ injected from tail vein. Observed rat's amounts of food, drink and urine;body weight, visceral fat weight and Lee's index. Detected FBG, PBG, FINS, FFA, TG, TC, HDL-C, LDL-C and blood rheology index by using biochemistry methods. Appraised IR by hyperinsulinemic-euglycemicclamp. Observed rat's histopathologic change of visceral adipose tissue, liver, kidney, pancreases, skeletal muscle and arch of aorta. Studied on the genome expression of adipose tissue around internal organs by gene chip technique.3. Insulin resistance rat model with the feature of type 2 diabetes mellitus was treated by Chinese drugs. Observed rat's amounts of food, drink and urine;body weight visceral fat weight and Lee's index. Detected FBG, PBG, FINS, FFA, TG, TC, HDL-C, LDL-C and blood rheology index by using biochemistry methods. Appraised IR by hyperinsulinemic euglycemic clamp. Observed rat's histopathologic change of visceral adipose tissue, liver, kidney, pancreases, skeletal muscle and arch of aorta. Studied on the genome expression of adipose tissue around internal organs by gene chip technique.Results1. Compare to the normal rat, the simple obesity model rat's BW, VFW and Lee's index increased (P<0.01);the FBG was no change, FINS and HOMA-IR increased (P<0.01), GIR decreased (P<0.01);FFA, TG and LDL-C increased (P<0.01), HDL-C decreased (P<0.01), TC and blood rheology index increased slightly. In histopathology, the volume of visceral adipose cells enlarged;liver cells had fatty degeneration;other tissues' were normal basicly. There were 115 different expressed genes. 85 genes were up-regulation;30 genes were down-regulation. They involved in lipid metabolism, energy metabolism, cell factor, inflammatory factor and immunity.2. Compare to the normal rat, the diabetes mellitus model rat's BW, VFW and Lee's index increased (P<0.05,P<0.01);the amounts of food, drink and urine increased (P<0.01);FBG, PBG, FINS and HOMA-IR increased (P<0.01), GIR decreased (P<0.01);FFA, TG, TC and LDL-C increased (P<0.01), HDL-C decreased (P<0.01), blood rheology index increased (P<0.01). In histopathology, areas of langerhans' structure became blurred, the amount of them decreased, the volume of them shrinked;the volume of visceral adipose cells enlarged;liver cells had fatty degeneration;the volume of renal glomerulus shrinked, part of them became stiffen;the structure of aortic arch was disorganized. There were 184 different expressed genes. 96 genes were up-regulation;88 genes were down-regulation. They involved in glycometabolism, lipid metabolism, protein metabolism, energy metabolism, signal transduction, cell factor, inflammatory factor, immunity and cytoskeleton.3. . Compare to the diabetes mellitus model rat, the treated rat's BW, VFW and Lee's index decreased (P<0.05,P<0.01);the amounts of food, drink and urine decreased (P<0.01, P<0.05);FBG, PBG, FINS and HOMA-IR decreased (P<0.05,P<0.01), GIR increased (P<0.01);FFA, TG, TC and LDL-C decreased (P<0.01), HDL-C increased (P<0.01), blood rheology index decreased (P<0.01). In histopathology, the structure of areas of langerhans was normal, the amount of them decreased slightly, the volume of part of them shrinked;the volume of visceral adipose cells was normal;the structure of liver, renalglomerulus, and aortic arch were normal. There were 2198 different expressed genes. 1160 genes were up-regulation;1038 genes were down-regulation. They involved in glycometabolism, lipid metabolism, protein metabolism, energy metabolism, signal transduction, cell factor, inflammatory factor, immunity and cytoskeleton. ConclusionInsulin resistant relates to various genes, involved in glycometabolism, lipid metabolism, protein metabolism, energy metabolism, signal trasduction, cell factor, inflammatory factor, immunity and cytoskeleton. The Chinese medical pathogenesis of insulin resistant is various pathologic matters stagnation in human body and functional disorders of viscera. The treatment method of removal pathologic matters and regulation the function of viscera is able to improve insulin resistant. The mechanism of it involves in regulation of intestine and stomach, reduction of visceral adipose tissue;improvement of various metabolism and protection liver cell;improvement prethrombotic state and protection blood vessel endothelial cell;regulation of cell and inflammatory factors;improvement of signal transduction.