A Study On The Expression Of E-Selectin And Its Mechanisms In Bladder Transitional Cell Carcinoma

Part â… Expression of E-Selectin and the potiented clinical implications in human bladder transitional cell carcinoma Objective: To investigate the expression of E-Selectin in human bladder carcinoma and go further into its possible clinical prognostic value. Materials and Methods: The block samples of 44 human bladder carcinoma and 9 normal bladder tissues in frozen, acetone fixed were examed with immunohistochemical method for E-Selectin, we want to find the correlation between the expression of E-Selectin and the pathological grade and clinical stage. Results: E-Selectin over-expressions was found in 26/44(59.1%) tumors. There were no E-Selectin expressions in the 9 normal bladder tissues. There was a significant correlation between the over-expression of E-Selectin and advanced clinical stage, but there was not significant correlation between the over-expression of E-Selectin and increased pathological grade. At T4 stage, E-Selectin's over-expressions was the most intense on the micro-vessels of the tumors. Conclusion: (1). The over-expression of E-Selectin is frequent in bladder transitional cell carcinoma. (2). There was a significant correlation between the over-expression of E-Selectin and metastasis and recurrence in bladder transitional cell carcinoma. (3). The over-expression of E-Selectin may be a potential prognostic marker in bladder transitional cell carcinoma. Partâ…¡A preliminary study of mechanisms associated with E-Selectin expression in bladder transitional cell carcinoma. Objective: We make a preliminary study on the expression of the E-Selectin mRNA. E-Selectin and NF-kBp65 and its possible mechanisms that lead to increased E-Selectin expression in bladder transitional cell carcinoma. Materials and Methods: 32 fresh surgical specimens of BTCC were examed by immunohistochemical method for E-Selectin protein and NF-kBp65 expression, in the meantime, a study was made for E-SelectinmRNA expression using hybridization in situ. We discuss the correlation among the expression of E-SelectinmRNA and E-Selectin and NF-kBp65. Results: 23/32 tumors have abnormal E-SelectinmRNA expression, 20/32 tumors show abnormal E-Selectin expression, only 1/32 tumorhave NFk-Bp65 over-expression. Abnormal E-SelectinmRNA expression was a significantly associated with tumor clinical stage but not with increased tumor grade; 18/32 tumors show abnormal expression both E-SelectinmRNA and E-Selectin. 1/24 tumor shows abnormal NF-kBp65 and E-SelectinmRNA expression together. There was significant correlation between the over-expression of E-SelectinmRNA and E-Selectin protein, but there was not significant correlation between the E-SelectinmRNA, E-Selectin protein and NF-kBp65. Conclusion: (1). There was a significant correlation between the up-modulation of E-SelectinmRNA and tumor's invasion in bladder carcinoma. (2). There may be multiple mechanisms that cause the abnormal expression of E-Selectin in bladder transitional cell carcinoma, the upward modulation of the mRNA may be one of the major mechanism with abnormal E-Selectin staining in bladder transitional cell carcinoma. There was not significant correlation between the abnormal expression of E-Selectin and the activation of NF-kB.