| Objective To study the effect of intracerebral transplantation of human P-NGF gene modified bone marrow stromal cells (BMSCs) from GFP (Green fluorescent protein) transgenic mouse to treat PD model rats. Methods Stereotaxically injected 6- OHDA into two points of one lateral corpus striatum was used to establish stable PD model rats.After cloned human β-NGF gene and established its eukaryotic expression vector pcDNA3-β~NGF, the recombinant plasmid pcDNA3-β-NGF was transfered into cultured and purified bone marrow stromal cells (BMSCs) of GFP transgenic mouse in vitro , and the resistant clones of stable expressing β-NGF under G418 selection was established.Then the β-NGF gene modified BMSCs and non-gene modified BMSCs were transplanted stereotaxically into the corpus striatum of the PD model rats. At different time after transplantation the rats were tested for apomorphine induced rotation behavior and the brains of PD model rats were examined with fluorescent microscope and immunocytochemical staining. Results Both β-NGF gene modified BMSCs and non-gene modified BMSCs survived in the brains of PD model rats, and showed good compatibility with the host cells.BMSCs integrated the brains of PD model rats, showed no signs of destroying the host and the glial cicatrisation.The β-NGF gene modified BMSCs expressed β-NGF stablely in the the brains of PD model rats, and showed obviously improving the rotation behavior of PD model rats induced apomorphine compared with non-gene modified BMSCs. Conclusion BMSCs is a kind of attractive candidate cell for the gene and cell therapy of Parkinson's disease, the intracerebral transplantation of β-NGF gene modified BMSCs can markedly improve the rotation behavior of PD model rats induced apomorphine compared with non-gene modified BMSCs.
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