Effects Of EMP And HPMW On Rabbit Optical System

Objective: Eye is very sensitive to electromagnetic radiation injury. HPM and EMP weapons, as well as electromagnetic radiation accidents will cause eye injury. The aim of this study was to explore the effects of electromagnetic radiation on eye: the pathological changes, the dose-effect relationship and its mechanism. Methods: The simulated HPM and EMP weapon device was used. Monkeys, dogs, rabbits, cultured cells were all irradiated for a long-term and dynamic study. Results: 1. Eye is very sensitive to electromagnetic radiation injury. HPM and EMP can induce eye injury in monkeys, dogs and rabbits. HPM and EMP-induced pathological changes are similar. The radiation dose and injury effect are positively related.2. Four animal models of eye radiation injury were set up. The sensitivity of eye tissue to radiation injury is as follows: lens>cornea>retina. The degree of EMP-induced injury in 3 kinds of animal eyes is very different: it is more serious in monkeys than in dogs, and it is more serious in dogs than in rabbits. It is suggested that EMR injury to eye is species different.3. Lens is very sensitive to radiation injury. Lens capsule membrane thickening, epithelial hyperplasia, lens fibers derangement and pigmenting uneven, lens edema and clouding, and finally cataract were all presented during 45 to 90 days after radiation. Clouding of posterior capsule of lens is easily found in monkeys, and clouding of anterior capsule is seen comparatively frequently in dogs.4. Radiation interfered proliferation and DNA synthesis in lens epithelial cells. In early days after radiation, AgNOR and DNA content was decreased; afterwards, when lens epithelial cells repaired from injury, the AgNOR and DNA content increased and cell proliferation was activated. Radiation-induced cell proliferation disorder plays an important role in cataract happening.5. Radiation interfered cell cycle of lens endothelial cells. Radiation-induced abnormal expression of p21WAF1 and cyclin E was involved in the change ofendothelial cell cycle. The abnormal expression of cell cycle regulating factors played a crucial role in radiation-induced cataract.6. Cell membrane is a very sensitive target for EMP radiation injury. Radiation may induce membrane perforation in cultured endothelial cells, and the ionic concentration in culture medium was increased obviously after radiation. Cell membrane injury plays a crucial role in the mechanism of non-heating effect caused by electromagnetic radiation.Conclusions:1. Lens is very sensitive to HPM and EMP radiation injury, and may lead to cataract;2. The radiation dose and injury effect are positively related;3. The degree of EMP-induced injury to 3 kinds of animal eyes is very different. EMR injury to eye is species different;4. Radiation-induced cell proliferation disorder plays an important role in cataract happening;5. The abnormal expression of cell cycle regulating factors played a crucial role in radiation-induced cataract;6. Cell membrane injury plays a crucial role in the mechanism of non-heating effect caused by electromagnetic radiation...