Pumonary Local Memorial Effect Of Lymphocytes In Asthmatic Airway Inflammtion

Objective To investigate if there is long-lived inflammatory memory in the lung with persistent asthmatic airway inflammation; and whether pulmonary local memory lymphocytes may transfer this kind of inflammation. Method 1 .Establish a murine model of asthma, and evaluate the inflammation and memory on 102 days postaerosol, including lung pathology, bronchial alveolar lavage (BAL) total cell counts and differential cell counts, IL-5 level in BAL, cell proliferation and IL-5 level in BAL cell and splenic cell culture in vitro. 2.Adoptive transfer BAL cells from asthmatic mice on 12 days postaerosol to naive mice, evaluating inflammation and memory postaerosol with the methods above. Results 1 .There are alveolitis and scattered vasculitis in the lung of asthmatic mice on 102 days postaerosol. After second OVA challenge, alveolitis and vasculitis increase rapidly, BAL total counts increase quickly and dramatically, eosinophil ratio increases significantly, but the IL-5 level in BAL is still relatively low. BSA substituting OVA for the second challenge leads to partial inflammation. 2.Transferring BAL cells free of macrophages to naive mice induces the lung inflammation like asthma, and the inflammation level is consistent with the infused cell number. Conclusion There is long-lived inflammatory memory in the lung of asthmatic mice model, and pulmonary local lymphocytes may play some roles in this process of memory.