Study On The Mechanism Of The Rule Of Strengthening Yang Invigorating Heart And Tranquilizing Preventing And Treating Atrial Fibrillation

Strengthening yang and invigorating-heart and tranquilizing is effective recipe to cure atrial fibrillation which professor CaoHongXin summarizes from long-term clinical practice.Utilizing the techniques, such as RT-PCR, immunohistochemistry, optical microscope, electron microscope, the paper investigates the effect of the regulating factors of atrial electrical remodeling(AER) and atrial structural remodeling (ASR) Cox40, Cox43, LTCC, ERK₂and prothrom botic state on atrial fibrillation (AF), meanwhile, observes the intervening effect of the rule of strengthening yang and invigorating-heart and tranquilizing.This paper studies the mechanism of AF and the rule on molecular level.The results show that:1, The heart weight index and AF duration increases in the AF mouses, the rule suppresses the myocardial hypertrophy, delay atrial muscular remodeling.2, The hemodynamics is abnormal in experimental mouses with AF, the rule improves the cardiac function through enhancing the level of LVSP and lowering the level of LVEDP and increasing cardiac output and lowering the heart rates, and improving the cardiac systole and diastole effectively.3, Ach-CaCl2 induces myocardial apoptosis.The rule inhibits apoptosis and myocardial fibrosis ,protecting the ultrastructure and decrease the motality. through antagonizing the abnormal activation of ERK₂, up-regulating the expression of Cox40, Cox43 mRNA and protein.4, The prothrom botic state is abnormal expressed in AF mouses, the rule suppresses the prothrom botic state, decreases the plasma concentration of GMP140, DD, TAT significantly.5, Ach-CaCl₂ induces the decreased expression of Cox40, Cox43 mRNA and protein in AF mouses.The rule up-regulates the expression of Cox40, Cox43 mRNA and protein, which maybe the therapeutic mechanism of inhibiting ASR.