Experimental Study On The Prevention Of Cerebral Vasospasm By In Vivo Endovascular Endothelial Nitric Oxide Synthase Gene Transfer

Objective To study new methods of prevent delayed cerebral vasospasm after subarachnoid hemorrhage, we transfect recombinant eNOS gene to SAH rat from endovascular path. Methods We established a recombinant adenovirus vector carrying endothelial nitric oxide synthase gene. We established delayed cerebral vasospasm rat model using double-hemorrhage model. Recombinant adenovirus was injected into carotid arterial to transfect the rat. Results Western analysis and immunohistochemistry detected recombinant eNOS mainly in endothelium of middle cerebral artery isolated from carotid-AdeNOS-transduced rats, and in this group NO and cGMP concentrations were significantly elevated by day 7. Analysis of day 7 versus day 0 middle cerebral artery diameter for each group revealed significant spasm reduce in AdeNOS-transduced SAH rats. Conclusions Our results suggest that expression of recombinant eNOS in the endothelium of cerebral arteries of carotid-AdeNOS-transduced rats may contribute toward relieving delayed cerebral vasospasm after SAH.