Transplantation Of Bone Marrow Mesenchymal Stem Cells Transferred With VEGF Gene Improved Cardiac Remodeling After Myocardial Infarction

objective To observe the effect of transplantation of bone marrow mesenchymal stem cells transferred with VEGF gene on cardiac remodeling after myocardial infarction. Method Model of myocardial infarction of rats was constructed. Bone marrow mesenchymal stem cells induced with 5-AZA in vitro were transferred with AdTRE. VEGF₁₆₅ and then were transplanted into infarct zone of rat's hearts. Heart function was evaluated with echocardiogram. Change of heart's morphology and capillary density was evaluated by histopathologic examination 4 weeks after operation. Change of collagen and MMPs was assessed by immunohistochemistry. Results Transplanted cells were differentiated into cardiomyocyte-like cells. Wall thickness of infarct zone and capillary density of heart were increased. Expression of collagen and MMPs was decreased. Heart function was improved significantly in rats injected with bone marrow mesenchymal stem cells transferred with AdTRE.VEGF₁₆₅. Conclusion Our data suggested that transplantation of bone marrow mesenchymal stem cells transferred with VEGF gene improved heart function and reversed cardiac remodeling.