| Objective To explore the possibility of the establishment femoral pseudoaneurysm (PA) and traumatic arteriovenous fistula(tAVF) models in rabbits. Models were analyzed by blood gas analysis, checked by colour doppler flow imaging(CDFI), examined by computed temography angiography(CTA), magnetic resonance angiography(MRA), digital subtraction angiography(DSA) and histological and transmission electron microscope observation. The experiment show the characteristics in the advanced diagnosising methods including CDFI,CTA, MRA, DSA and so on .At the same time ,It provides a good chance for the diagnosing instruments Methods To establish PA,tAVF models in 40 rabbits by microsurgical technique,male or female are not concerned,their weight are around 2.5 to 3.5 kg and they were divided into two groups in same amount. (1) PA group: Anaesthesia: 2.5% sodium pentobarbital was injected into the vein of ear at 30 mg/kg. Followed by fixation ,skin preparation ,sterilization and other preparation and then a skin incision was made about 4 cm long in femoral region to expose the femoral artery,block the blood flow with Bull dog. made a 3 mm long incision on the femoral artery On the oppositing muscle wall of the incision was made a 3 mm hole in diameter with ,Anastomosis were made with 9-0 Dacron suture by microsope The operation finished until no bleeding in the site and the distal artery pulse could be palpated. The animals were sent to animal center to breed . they were divided into 5 teams in average . (2) tAVF goup: the anaesthesia and preparations were same to the group(1). exposing the femoral artery and vein .blocking the blood flow with Bull dog, made a 3 mm long incision on the femoral vein , on the femoral artery, a 3 mm×1 mm ellipse hole was made and anastomosis can be done with 9-0 dacron suture between the artery and vein,at the end the model was finished. tAVF model was established ,they were divided into 5 teams in average ,30 tAVF operations were made in the group . Models were analyzed by blood gas analysis, checked by color doppler flow imaging(CDFI), examined by computed temography angiography (CTA), magnetic resonance angiography(MRA), digital subtraction angiography(DSA) and histological and transmission electron-microscopy observation. Results It was possible to establish PA and tAVF models with micro-surgical techniques .the surgical skills play an vital important role during the experiment.16 PA models were made in rabbits(16/20,87%). 30 tAVF models were made (100%). The time of Pulsating masses occured in PA group around 5~11 days, and the most appeared around the 7~9 days .In the tAVF group ,there were established at the end of suture. Each testing method has its advantages and disadvantages: I . CDFI It can provide information of anatomy and function in extremities vessels,while in diagnosising of peripheral vessel diseases,much more special theory and technique are needed than regular manipulation . ①PA group :In the two-dimension picture,round masses could de found in the femoral region ,the diameter varied from 0.5 to 5.5 cm.The masses had clear borderline ,and the wall of PA was thick .In big one ,irregular thrombus with moderate echo could be found .Sometimes no echo in the mass ,while increasing the gain moderately , eddied flow in small dense echo could be found . CDFI revealed color mosic in the mass .Sometimes supplying artery could be shown. In the systolic period ,colorful flow ejected into the cavity and whirled in it ; in the diastolic period ,the blood flowed back into the artery. The blood flowed in and out with regular rhythm. In the Dopplar velocity of flow curve ,bidirection blood flow could represent. At thecrevasse ,the blood flow could be measured with high speed and resistance in systolic period ,and reverse low speed and resistance flow in diastolic period .In the cavity ,the whirl flow was very slow . ②tAVF group :the hole between the vessels in tAVFs was not founded in two-dimension picture, but we can guess the site: In the artery , the inner diameter o... |
PDF Full Text Request