Experimental Study On Transfecting Gene Into Bone Marrow Mesenchymal Stem Cells To Develop Tissue Engineering Heart Valves

Objective: To investigate method promoting the adhesion of seeding cells. Methods: (1) Prokaryotic expression, extraction and purification of FnCBD₆₄. (2) Construction of recombinant adenovirus coding for FnCBD₆₄ gene. (3) Isolation, culture of BMSCs and expression, secretion of FnCBD₆₄ after transfection. (4) To study enhancing the adhesion of BMSCs after precoating FnCBD₆₄ on scaffold or transfecting Ad.FnCBD₆₄ into BMSC_s Results: BMSCs can express and secrete FnCBD₆₄ efficiently after transfected. There were more BMSC_s in a well of 96-well plate after precoated. The more BMSCs adhered on a acellular leaflet constructed with precoating or transfecting methods and BMSC_s formed a continuous multilayer closely on it. When underwent higher flow for 6 hours, leafet with same ways constructed from whole aortic roots had more residue cells, moreover much more in Ad.FnCBD64 group.Conclusions: Precoating FnCBD₆₄ can promote adhesion and proliferation of BMSCs on decellular aortic valves, transfecting Ad.FnCBD₆₄ make better effects.