Study Of The Preconditioning Combination Of Oral Administration Of Geranylgeranylacetone And Local Somatothermal Stimulation For Conferring Tolerance Against Ischemia-reperfusion Injury In Rat Livers

AIM: to explore a simple, effective, safe and operable pretreatment strategy for conferring tolerance against ischemia-reperfusion injury after liver surgery in rat livers. METHODS: forty-five rats were divided into five groups (each group n=9). Group C: control group; group G: GGA was administrated without heat stress; group S: local heat stress alone was perfurmed; group WG: GGA plus whole-body heat stress was perfurmed; group SG: GGA administration plus local heat stress was perfurmed. After completion of the liver ischemia-reperfusion procedure, the ischemic-reperfused liver lobes in five groups were resected and tested for HSP70 by PT-PCR, Western blotting analysis and immunohistochemical staining. The blood samples were collected for ALT and AST measurement at the end of occlusion of blood supply, 30 min after reperfusion, 24, 48, 72h after surgery. Survival was...