Screening And Identification Of The Differentially Expressed Proteins In Brain Stem Of Seasickness Susceptible Rats

Objective: To study the differentially expressed proteins in brain stem of seasickness susceptible rats and discuss its possible pathogenesis. Methods: Transmission electron microscopy was used to study the ultrastructure of seasickness susceptible rats. The total brain stem proteins were isolated and identified by two-dimensional electrophoresis. Peroxiredoxin I was confirmed by Western Blot. The activities of T-AOC, SOD, ROS were also examined. Results: The ultrastructure changes of seasickness susceptible rats were in close relationship with vestibular hypoxic-ischemic changes of brain stem. The differentially expressed proteins were related to the decreased energy metabolism, increased oxidation stress, signal transduction failure, dysfunction of neurotransmitter and iron metabolism et al. T-AOC and ROS of brain stem decreased while reactive oxidative species increased. Conclusions: Seasickness susceptibility, which was in relationship with many aspects, was a complex procedure resulted from brain stem hypoxic-ischemic changes provoked by seasickness stimulus. Oxidative stress played an important role in the development of seasickness although it might not be the pivotal pathogenesis.