Comparison Between Mobilization And Transplantation Of Bone Marrow Stem Cells For The Therapy Of Myocardial Infarction In Rabbits

Objecive:To compare bone marrow stem cells mobilization with bone marrow-derived mononuciear cells (BMC) transplantation for the therapy of myocardial infarction (MI) in rabbits, explore more effectively and practical stem cells therapeutic strategy for MI . To isolate endothelial progenitor cells(EPC) and mesenchymal stem cells(MSC) from the mobilized peripheral blood and transform MSC into cardiomyocytes,establishing a new and non-invasive cell source of stem cell transplantation for MI therapy.Methods: 30 rabbits were divided into 3 groups randomly.In mobilization group(group M), granulocyte-colony stimulating factor (G-CSF)( 30μg.kg^-1.d^-1) was injected subcutaneously 3 hours after MI and every 24 hours for 5 days.On the 5th day the mononuciear cells(MNC) from 10 ml peripheral blood were labeled with bromodeoxyuridine (Brdu) for 24 hours, then reinjected intravenous.In transplantation group( group T), BMC transplantation was performed 5-7 days after MI. After being obtained from bone marrow (3⁵ml) of iliac crest and labeled with Brdu for 24 hours, MNC were transplanted into infracted myocardium through intramyocardial injection. Control animals ( group C) didn't receive any treatment after ML Echocardiography were performed for the evaluation of cardiac function 1 week and 5 weeks after MI, hemodynamic studies and histological study were performed 5 weeks after MI. Endothelial progenitor cells(EPC) and mesenchymal stem cells(MSC) were isolated from peripheral blood after being mobilized by G-CSF. After being treated with 5-azacytidine for 24 hours,the cultured cells and EPC were identified.Results: LV ejection fraction of group M increased significantly , group...