The Effect Of Dsl Or Dsl Extract With CsA On Transplanted Heart

Objective: Creat the cervical heart transplantation model between Wistar and SD rats. To induce the donor specific immunologictolerance, the recipient was injected with donor lymphocyte and extract of donor lymphocyte after the heart was transplanted. Cyclosporin-A was injected into abdominal cavity of recipient after operation. Observe the survival time of donor heart and grade of heart rejection.Material and Methods: Wistar rats were used as the donor and SD rats as recipients. Body weight of Wistar range from 150 to 250 grams, and the SD range from 250 to 350 grams. The rats are devided into 5 groups. Group A included 5 donors and 5 recioients. Group B included 15 donors and 15 recipients. Group C included 15 donors and 15 recipients. Group D included 15 donors and 15 recipients. Group E included 5 Wistar as donor and 5 Wistar as recipient. 10% chloral hydrate was injected into the donor's abdominal cavity to anesthesia it. Separate the right external jugular vein and common carotid. Deligation the axifugal terminal and cannula the other terminal. Anesthesia the recipient, abdominal median incision. The recipient was heparinizationed all over the body. 5 ml blood was drew from recipient abdominal aorta. Cut the sternum. Inject 10 ml 0℃ beat-ceasing fluid through descending aorta to cease the beat of donor heart. Cut off and deligation the inferior vena cava, left superior vena cava. Cut off descending aorta. Separate the heart, lung, esophogus as a whole part along the spinal column. Clip the donor heart and connect the pulmonary artery of donor heart with jugular vein of recipient, connect the aorta of donor heart with common carotid of recipient. Open the vascular clamp and the donor heart rebeat slowly. Several seconds later the rhythm became irregular and fast. Group C recipient was injected with donor lymphocyte 5 × 10~7 through caudal vein after the donor heart was transplanted. Group D recipient was injected with donor lymphocyte extract 0.5 ml through caudal vein. Grading of heart...