| Endometriosis(EMS), the present of endometrium outside of the uterine cavity, is a common disease, causing abdominal pain,dysmenorrhea, dyspareunia, and infertility in 10-15% of menstruating women. Endometriosis is considered to be an estrogen-dependent disease. A variety of hormonal treatments for endometriosis have been used, including drugs such as danazol, progestogens, gestrinone, prostaglandin synthetase inhibitors and GnRH analogues in the treatment of endometriosis comes from comparative with danazol. Many studies have investigated the effect of GnRH analogued in comparison with danazol. Studies show that GnRH-a is the most effective and the most promising medicine for treating endometriosis. It is estimated that approximately one third of the women who complain to a physician of pelvic pain or infertility have endometriosis and that its incidence is incerasing. Endometriosis remains an enigma among women's diseases and presents many problems regarding its treatment and prognosis due partly to the great variation in the severity of the disease. Although EMS is a benign gynecological disease,its biological action is similar to malignant tumor .The formation of new from existing blood vessels is essential for tumor growth, invasion and metastasis. VEGF is one of the vasual inner epithelial cells leading to new vascular formation.Matrix metalloproteinases(MMPs) play an important role in degradation of extracell matrix and basement membrane components . MMPs are highly homologous protelytic zinc enzymes responsible for degradation of extracellular matrix components,such as collagen,proteolycans,fibrotectin and laminin. MMP-2,which degrades type IV collagen,may play a role in tumor metastasis.Materials and Methods: A total of 80 animals underwent the procedure for induction of endometrisis. We chose 11 rats as the case-control group randomly. We chose 11 rats as the operation group randomly and draw the uterine not making endometrium transplant. The rest of 58 animals underwent the surgery operation. Then three pieces of 5×5mm fragments of endometrial tissue were transplanted to different places；We observe and measure the length,width and height of the implantion and count each volume of them and compare the volume at the forth, sixth and eighth week after the operation.We use the light telescope to observe the histology of ectopic endometrium to make sure that the transplant is endometrium. Among them there are 34 rats,which are successful in endometrium transplant.Rats with experimental endometriosis were randomly divided into 3 groups:1 Group LHRH-PE40 :12 rats,15ug/kg /d,im.continuly for one month.2. Group Naf : 11 rats,5ug/kg /d,im.continuly for one month.3.Model group:11 rats,not giving any medicine and raising them normally. To kill all the rats by pulling the heads and they were laparotomized. To compare the size and structure of the transplant endometrium by naked eyes and lightelescope.Measuring the level of the E2 ,FSH,LH and PRL by radioimmunoassay. VEGF and MMP-2 in eupotic and ecpotic endometrium were measured by immunohistochemitry method. We use RT-PCR to compare the effects of Nafarelin and LHRH- PE40 on endometriosis respectively.Because cyclic estrogen and progesterone are the primary stimuli for endometrial growth and remodeling, medical therapies for endometriosis have included attempts to suppress steroid action. Gonadotropin-releasing hormone agonists that cause a reversible block of ovarian cyclicity have been used in the mangement of endometriosis. Results: 1.There are 34 rats in the 58 rats which are successful in creating models and successful rate is 59%. Eutopic endometrium is becoming larger at first and then becoming smaller with the time of the transplant. The volume in uterin ligament is much larger than in the abdominal wall at the same time and the difference is significant(P<0.05).There is no significantly difference on the eutopic endometrium volume between the abdominal wall and the crossing site of the uterine(P>0.05...
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