| Purpose With the age getting older and older in population in our country,the number of the Alzheimer 's disease reports show that the degenerative neuro pathy diseases as such AD is closely related with oxidizing which should add the oxidizing injure of the protein and DNA and cause the death and dysfunc tion of the neuron.But the researchs have developed a new way to treat these degenerative neuropathy diseases. Melatonin is a kind of hormone secreted by conarium.The present reports indicated that it can clear the free radicals, incre ase the activity of anti- oxidize enzyme and decrease the toxicity of the Aβof the neurons and regulating living creature clock and neuron immunity, ect. The melatonin has the high lipoid dissolution and the low combination to blood plasma portent ,so it can pass the blood brain barrier very easily .Therfore, the melatonin has thought is a kind of treatment to AD.The main functions of the melatonin: anti-free radicals function: Directly clear free radicals,inhibit the reaction of the over oxidizes to the lipoid, increase the activity of the other anti-oxidizes enzyme and the genes`expresses; regulates the immunity the function, regulate the enfocrie function;Other functions: the MT has the various living creatures fuction,such as regulating the circadian rhythm,repressing secretes the sex hormon and growing the hormone,regulating the stress reaction,and anti-decrepitude etc.But it has not been reported in the domestic and international that the concrete function atits different density.This study examined the mechanisms of oxidative βstress induced by A? and the mechanisms of anti –oxidative role by melatonin. MethodsThe lesson includes two parts: the animal model and the body side cell model, using AD rats model in the animal study and checking the study and memory ability of the model rats, it has been proved that the model rats is examine the influence of the decrepit brain successfully which is hurt by the oxidizing,and the protection function of the melatonin based on the biochemistry method examination and immunohistochemical dye. In the cell research we apply D- galactose induce P12 cell hurt model and A β 25-35 induce divide of PC12 cell hurt model to research the ant-oxidize hurt of the melatonin the results indicate that the β –amyloid and D- galactose maybe arouse oxidize hurt, while the melatonin has active function.A, the establishment of the D- galactose decrepitude model, the observation of changes in behavior, the good action of melatonin Using the water labyrinth test, we divided the rats with quickly reaction into five groups in random, each group 12 rats. ①physiology brine group;② A β group;③ D-galctose group;④ A β + D- galctose group;⑤ melatonin group.In the ③④groups,each animal belly cavity was injected with the D-galactose:50 mgs/ kg.d,with continued injection until 6 weeks, the decrepitude animal model established . In the ①② groups each animal belly cavity was injects with same quantity physiology brine.In the 7th week, the ②④ groups were injected with β- AP into rat's hippocampal region, 1 μl in each side; andthe groups of ①③ were injected with same quantity physiology brine.The ⑤groups were injected with the same thing as the group of ④ into the belly cavity and the double hippocampal region ,which being injected with melatonin 5 mg/ kg.d.into the belly cavily at the same time.After establishing model successfully, in the first,second and fourth week , we used the water labyrinth test to observe the changes of the rats behavior.observeing the appearance of the neuron in hippocampal with HE staining.observing Missl body with MisslStaining,observing the super microstructure mitochondrion in hippocampal region with electron microscopy. examining SOD activity and the contain of MDA in hippocampal and frontal lobe in the biochemistry method ,examing the expressions of protein Bcl-2 and GFAP in hippocampal region and frontal lobe of AD big in immunohistochemical mothed .2,we used MTT m...
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