The Related Research On The Application Of The Antitumor Active Artificial Bone In The Spine Tumor

Spine tumor is one of the most important disease in the spine surgery field. With the advance of the idea of the surgery treatment and the technique, Surgery treatment has being gradually become the important method. However there are particular concerns with spine tumor. It is difficult to remove tumors with en bloc or wide resection, as some important structure in the adjacent of the spine, spinal cord , large vessels and some important viscera for instance. The realistic choice in the clinic practice is performing the margin resection even intralesion resection reluctantly. This is one of the main causes for the postoperative recurrence of many spine tumors. On the other hand, the effective treatment methods are often lacked in the recurrence spine tumor.It has shown that the Calcium Phosphate Cement (CPC) has the role of slow time-release and can provide the reliable stiffness and strength in the application of the vertebroplasty. When the CPC compounded with drugs ,its mechanics capability will decrease. To keep the mechanics characteristics of the CPC, the content of the drugs in the CPC shouldn't too high and it can bring into excellent play in the very low concentration.The investigations have shown that As2O3 has a role of inducing apoptosis in varieties of tumors and the inhibitory rate often higher than many anti-tumor drugs. The clinc researches have shown that it is effective for the obstinate or recurrent tumors with the fewer side effect.The following seven experiments had been undertaken:1. Research on the properties and the release characteristics in vitro of the compound antitumor CPC (CAC). Studing the CPC solidation and properties, it has shown the powder of As2O3 and MTX compounded with CPC in the ratio of 5% can fit for the clinic appliance.2. Research on the in vitro release of CAC. It has shown that the mechanics strength of the As2O3 and MTX CAC strengthen after slow release.3. The preparation of the As2O3 albumin microsphere and the research on itsproperties. In order to As2O3 CAC can reach more ideal release effect and be beneficial to bone ingrowth with the micropores, construct the As2O3 albumin microsphere through emulsification-heat-solidation theory, and conduct its property research.4. The preparation of As2O3 albumin microsphere CAC and the research on its property. It has shown that As2O3 albumin microsphere compounded with CPC in the ratio of 1% is reliable. The release rate of As2O3 albumin microsphere is 9.22 times faster than that of As2O3 albumin microsphere CAC. After the release, the strength of the AS2O3 albumin microsphere CAC is strengthened.5. The inhibitory role of the CAC on tumor cells.Compare the inhibitory role of the As2O3, MTX, EPI on the entity tumor cells. It has shown that As2O3 has the 50% inhibitory role on the variety of tumors cells at the concentration of 6.25ug/ml.When the osteosarcoma cell cultured with the solution of the As2O3 CAC releasing test in d2,d5,d7 , it has shown the satisfactory inhibitory ability.6. The research on the influence of CAC on the spinal cord function. Observe the influence of the As2O3 albumin microsphere CAC, AS2O3 albumin microsphere and MTX CAC on the spinal cord. It has shown that As2O3 albumin microsphere CAC and MTX CAC can be put into clinic practice with slight side effect.7. The in vivo research of the CAC on tumor. Implanted the As2O3 albumin microsphere CAC and MTX CAC into lung model of the nude mouse. It has shown that the As2O3 albumin microsphere CAC has the stronger ability of inhibiting the growth and inducing the apoptosis of the of the tumor cell.The new ideas of this study:1. Discuss the feasibility of CAC on the spinal tumor.2. Construct the As2O3 albumin microsphere for the first time ,which has the wide application future.3. Compounded the As2O3 albumin microsphere with the CPC for the first time and carried out the basic research.