Ⅰ.Association Study Of Human HL Polymorphisms With Serum Biochemical Traits Ⅱ.Construction And Preliminary Screening Of Rat Hepatic Hypercoagulation-associated CDNA Libraries

823 Chinese Han adults aged over 40, including 466 men and 357 women were enrolled in the study to examine the association of +1075C and -480T polymorphisms in hepatic lipase gene (HL) with plasma lipoprotein and apolipoprotein levels. In this population the allele frequencies for HL +1075C and -480T were 0.053 and 0.362 respectively and the prevalence of HL +1075C/C and -480T/T were 0.006 and 0.132, respectively. Overall, the normal HDL-C (>=35mg/dL) subjects had a higher carrier frequency of HL +1075C than the low HDL-C (<35mg/dL) subjects (0.108 vs 0.029, P=0.039). However, when tested separately, the carrier frequency of HL +1075C was not significantly different between normal and low HDL-C females (0.101 vs 0.083, P=0.843). In males, the normal HDL-C subjects had a higher carrier frequency of HL +1075C than the low HDL-C subjects (0.113 vs 0.018, P=0.026). No significant difference of frequencies of HL -480T genotypes -480T/T, -480C/T and -480C/C was found between normal andlow HDL-C subjects. Among plasma TG, TC, HDL-C, apo AI, apo All, apo B100, apo CII, Apo CIII and apoE, only HDL-C and apo AI were significantly different among the three genotypes +1075A/A, +1075A/C and +1075C/C in men (.P=0.029 and 0.032). No association was found in women. Male subjects with +1075C/C had a higher level of HDL-C than those with +1075A/C (P=0.020) and +1075A/A (P=0.013), +1075A/C higher than +1075A/A (P=0.017). Male subjects with +1075C/C had a higher level of apo AI than +1075A/C (P=0.013) and +1075A/A (P=0.019), +1075A/C higher than +1075A/A (P=0.021). Although not so significant (P-0.053) as HDL-C and apo AI, male subjects with +1075/CC had a higher level of apo All than those with +1075A/C and +1075A/A. No significant difference of lipoprotein and apolipoprotein traits was found among the three -480T genotypes -480C/C, -480C/T and -480T/T, in the sample overall and in men and women separately. To further explore the association of HL polymorphisms with biochemical traits, 292 subjects (185 men and 107 women) were randomly selected from this population to examine whether association exists between the HL -480T polymorphism and the distribution of apo AI in HDL subpopulations. In this tested sample, the allele frequency for HL -^480T was 0.385. Of age, BMI; plasma TG, TC; HDL-C, LDL-C; apo AI, apo All, apo B100, apo CII, Apo CIII, apoE; alcohol consumption; percentage contents of apo AI in HDL subpopulations pre $ i-HDL, pre J3 2-HDL, HDL3c, HDL3b, HDL3a, HDL2a> and HDL 2b to total apo AI in plasma, no significant difference was found among the three -480T genotypes -480C/C, -480C/T and -480T/T, in the sample overall and in men and women separately. These results indicate that HL +1075C, not -480T polymorphism is associated with plasma high densitylipoprotein cholesterol and apolipoprotein AI in men in this Chinese population, HL -480T polymorphism is not associated with the distribution of apolipoprotein AI in HDL subpopulations of this Chinese population.