The Research Of Urapidil On Spinal Analgesic Aspect In Rats

ForewordUrapidil is a anti - adrenergic alphal receptor agent, usually used to cure hypertension. Its machenism is to block post - synaptic adrenergic alpha receptor and result in the obvious descentment of peripheral vessel resistance. On the other hand, by activating the 5 - HT1A receptor in the central nervous system, urapi-dil decrease the level of sympathetic feedback of cardio - vascular regulation center of brain - stem and depress the high blood pressure.The dorsum of spinal cord involve in amount of 5 - HT1A receptor, which make an important role in nerves conduction of pain,it takes part in not only the conduction of ascending nerve's pulse but also the conduction of descending nerve's inhibition.Detection of the FOS immunological reaction is a powerful marker to determine how much the injury of nerves is and the extent of analgesia. SP, as the matter released from termination of primary afferent nerve, take a very important role on the aspect of convection and regulation of harmful irritating information. Detection of the SP immunological reaction is an important method judging the analgesic effect.This experiment is a contrast research of myelo - analgesia of urapidil with the medium of extracellular electrophysiological technique , biochemistry cellular immunity technique and transmitting electron microscope, by adding influence actor of clonidine, ondansatron and morphine. The purpose of this research is to try to find out it's mechanism and interaction.Materials1. Experiment animal:Wistar's rats 250 -320mg(department of experimental animal, Med Uni)2. Main instrument:Image analyzer system ( Quantime970, Cambridge Ins) Multi - ways of physiological recording machine ( PM6000, NIHON KO-HDEN)Stat -thermometer box( Shanghai medical instrument factory) Transmitted electric telescope(1200 EXs, USA)3. Primarily medicine:urapidil (Xian pharmaceutical factory) clonidine( Peking pharmaceutical factory) ondanstron( Hengshui pharmaceutical factory) morphine ( Shenyang first pharmaceutical factory) anti - FOS ( Boster creature co. Ltd) anti - SP ( Boster creature co. Ltd)MethodsThe experimental animal, Wistar' s rats are divided into 8 sets by drawing lots:(1)normal contrasting group,no any irritating factor is given, drawing the materials of spinal dorsum after instillation; (2)saline group, long - term electricity irritation is given, drawing the materials of spinal dorsum after instillation; (3) urapidil group, long - term electricity irritation is given, drawing the materials of spinal dorsum after instillation ; (4) clonidine group, experimental condition as the saline group; (5) ondanstron group, experimental condition as the saline group; (6) urapidil + clonidine group, experimental condition as the saline group; (7) urapidil + morphine group, experimental condition as the saline group; (8) urapidil + ondanstron group, experimental condition as the saline group.Method A; Exert the rat into intra - abdominal aneasthesia state under 20%Ethyl urethan 1. 0g/kg . Put a Wistar's rat in prone position,take median incision , detach the subcutaneous tissue to spinous process, expose L1-4 Lamina ofvertebra under microscope, do away with the Lamina of vertebra, reveal L, ₄ spinal cord and cover it with warm salt water carbasus. By means of injection syringe , Dribbling the experimental durg onto spinal cord surface, Freeing and exposing sciatic nerve, lining plastic slot insulating the subcutaneous tissue, Placing stimulating electrode on the sciatic nerve;on the other hand, inserting the recording electrode vertically inside the dorsal root with the tungsten filament mi-croelectrode on the level of L1. Setting electrostimulating parameter as cycle -phase 34ms,wave - broadth 100 S, electric voltage 8v and recording the data with the PM6000 multi - physiological instrument.All data indicates with x s, and exerts pair - matching and inside - bundle t test to statistic analysis.Metho B: Exert the rat into intra - abdominal aneasthesia state under 20%...