Study On The Related Genes Of Parkinson Disease

[Background] Parkinson disease (PD) is a neurodegenerative disorder, whose Pathological hallmark is prominent loss of doparminergic neurons in the substantia nigra. The pathogenesis of PD is unknown. Epidemiological studies have identified exposure to environmental toxins, such as pesticide, insecticide and herbicide as risk factors for PD. The ability to inactivate toxins of individuals correlates with different levels of detoxification enzymes, which is determined by polymorphisms of genes encoding those enzymes. So genetic polymorphisms of detoxification enzymes may be associated with geneticsusceptibility to Parkinson disease. N-acetyltransferase(NAT) acetylate numerous chemicals containing aromatic amine or hydrazine groups, which are often found in herbicide, insecticide and dyes. So genetic polymorphism of N-acetyltransferase may have related with genetic susceptibility to Parkinson disease.[ Objectives ] To investigate the relationship between Parkinson disease(PD) and slow acetylator alleles and slow acetylator genotype of N-acetyltransferase.[Methods] Using PCR-RFLP, we compare the difference of three slow acetylator alleles Ml, M2 , M3 and slow acetylator genotype between 100 idiopathic PD and controls.[Results] Between whole PD and controls, the difference in frequency of slow acetylator alleles and genotypes wasn't significant(P>0.05)0 But for 54 early-onset Parkinson disease(onset<55y), the frequency of alleles Ml, M2, M3 were 6.5%, 26.9% and 22.2% respectively, while 4%, 16% and 16% in controls. The differences were statistically significant(P<0.05). The frequency of slow acetylator genotypes were 24.1% and 11% respectively for early-onset Parkinson disease and controls. The differences were statistically significant(P<0.05) too.[Conclusions] NAT2 Slow acetylator alleles and slow acetylator genotype of N-acetyltransferase might at higher risk to develop early-onset Parkinon disease.