MDR1 Gene Transfection To Improve The Effect Of Hepatocarcinoma Mice High-dose Chemotherapy

PART ONEObjective To investigate the protection effect of MDR1 gene transfection hematopoietic stem cells into marrow during the high-dose chemotherapy in Hepatocarcinoma Balb/C mice,and improve the curative effect.Methods Cultivated H22 hepatocarcinoma cell was injected into the balb/c mice to establish the animal model. MDR1gene was transferred into hematopoietic cells of murine bone marrow by the mediation of retrovirus vecter and transplanted into the balb/c mice with hepatocarcinoma which were exposured to 60Co-γray in advance. WBC of these mice were messured in the third day after high-dose chemotherapy. The function and the expression level of MDR1 gene on marrow HSC wrer detected by PCR method and IC method in vitro and in vivo. Results WBC of mice in different dose experimental groups was higher than that of control group significantly(p<0.01).There was no diffence between the experimental groups and the control groups in the first week.(p>0.05),but in the second week WBC counting of 0.8mg/kg group was lower than that of the other two groups(p<0.05).Mortality rate of 0.4mg/kg and 0.8mg/kg groups was lower than that of control group(p<0.05).p-gp positive staining rate of experimental group was 8.63±0.36%,while the control group was zero.Conclusion MDR1 gene transfection into bone marrow HSC in the Balb/C micecan pertect the bone marrow of these mice against chemotherapy and extend their survival time.