Gene Expression And Its Clinical Research Of Insulin-Like Growth Factor-1

?1 Effective Expression of Human Insulin Like Growth Factor-1 in Bombyx Mori LarvaeObjective To study the efficient expression and production of biologically active recombinant human insulin-like growth factor 1 (rhIGF-1) in silkworm system by the vector of Bombyx mori Nuclear Polyhedrosis Virus (BmNPV). Methods A full length 1GF-1 cDNA with the start codon at position -25 was inserted into the BmNPV genome under the control of the polyhedrin promoter. Silkworm larvae (JY1) were used as hosts for the infection with recombinant BmNPV /IGF-1. rhIGF-1 concentration were detected by ELISA, and Western blot was used to find the size of IGF-1, while MTT pathway was involved to study the bioactivity of rhIGF-1 by stimulation the growth of NIH3T3 cell line. Results It was found that from 72h to 120h post infection, the concentration of IGF-1 in silkworm hemolymph was gradually increased (19.09-23.74 u g/ml), and mature rhIGF-1 with a size of 7.5 kd was identified by Western blot analysis. It was also showed that rhIGF-1 has excellent bioactivity by stimulation of NIH3T3 cell growth. Further verificationwas per/ormed by specific inhibition of the biological effects using IGF-1 receptor antibody, IGF-1R a Conclusion Human IGF-1 was highly expressed by BmNPV expression vector, this production showed satisfied both in bioactivity and immunological activity thus providing a very efficient way to produce soluble and biologically active mature rhIGF-1.