| Wound and various kinds of disease caused larynx or trachea! cartilage defect are influencing patient's quality of life and threatening their life, therefore, laryngology doctors devote themselves to explore and consummate satisfied larynx, trachea of dissect and function characteristic method for repair and rebuild for many years. Autologous, allogeneic cartilage and artificial materials had been used in clinical, but there's demerit such as anatomic structure, supply border defect, immunization exclude and hard to mix together with body. It's possible to avoid defect of tradition cure method, and solve the diffculty of larynx and trachea of repair and rebuild as the development of tissue engineering technology. There need great quantity of seed cells and fine cytoskeleton materials for tissue engineering to satisfy the request of engineering tissue or organ for building and moulding.Culture of seed cells is a basic element of tissue engineering, cells mainly come from self-celK variant of the same homogeneity ^ cells of heterogeneity, and so on. Self-histiocyte is the first choice. Owing to tissue engineering often demands high concentration of inoculation cells, self-histiocyte has some questions about quantity of localization, passed culture for long period of time and function aging. So, this test was about to study the engineering of stem cells. The engineering of stem cells was to regeneration of mesenchymal tissues such as bone, cartilage, muscle, ligament, tendon, valve, adipose, derma, stroma, nerve, blood vessel, skin, cornea, as well as hematopoietic and immunity tissue by using modem biomedicine and tissue engineering technique, get across to separation purification direct induction> differentiation transfer gene and transplant nucleus > a great deal of expand increase and conformity construct in mesenchymal progenitor cells, embryonic progenitor cells, blood vessel/ hematopoietic stem cells , nerve stem cells and derma, muscle progenitor cells in vitro. Study on the progenitor of tissue engineering which have important role and deeply effect on the repair or replace of tissue and organ in the future. Compared with traditional tissue engineering, tissue engineering have obvious difference and advantage over its source of tissue and organic cells, cells expand and directional inducement differentiation, sort of reconstruct tissue and organic, efficiency of reconstruct, plant the reconstruct tissue and organic. Marrow mesenchymal stem cells are a study hotspot. The human MSCs have the ability to proliferate extensively, and they maintain the ability to differentiate into multiple cell types or their terminal phenotype in vitro. There are some MSCs in adult marrow, it can turn into many mesoblast tissue by differentiation, including bone and cartilage tissue, tendon, muscle, fat and marrow stroma tissue. In the course of cells culture, we can using the characteristic of MSCs who grew adherent, hematopaietic stem cells can be clean out and MSCs can be differentiated and purified when the medium ischanged. In this test, human MSCs was cultured and inducted into cartilage cells which could be used to repair and rebuild function in the field of Otolaryngology by using this characteristic of those cells. Based on this, we could repair someone's damaged tissue in systemic of catilage by using his own MSCs. Now, the assume are getting much attention and recognized by more and more people.Materials and Methods1. Differentiation of MSCs from human bone marrowHuman bone marrow was obtained from ribs of some in-hospital patients in department of chest surgery in our hospital.Low-glucose Dulbecco modified Eagle medium(L-DMEM) with 100 units/ml of penicillin and lOOug/ml of streptomycin was aspirated into ribs, the rinse liquid was collected, and a cell pellet was produced by centrifugation at 1000 rpm for 5 min. The supematants and fat layers was removed. The pellet was suspended in 15 milliliters of L-DMEM. It was fractionated on a Percoll gradient(70 percent initial density). The...
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