| Experimental study on apoptosisfrom portal hypertensive gastropathyAbstractThere are three parts in our study. The effects of disconnectionand shunt on the apoptosis from portal hypertensive gastropathywere evaluated. Each part's abstract described below:First Part: Experimental model and pathogenesis of portalhypertensive gastropathy with partial portal vein ligationObject To establish an experimental model of portal hypertensivegastropathy (PHG),which can be induced easily in short time.Methods Partial portal vein ligation(PVL) method was used tonarrow the sectiona1 area of portal vein by 90% to establish anexperimental model of PHG in 72 wistar rats. The opening ofarterio-venous shunts (A-V shunt) of gastric wall in the rats wereobserved by due dye injection at different postoperative time pointsand counted. Results Postoperative A-V shunt number was.increased significantly from(l l .28 f l .34)/cm' to (33. l4 l 3.52)/cm'at the first day (P<0.0l ), and reach the peak (42.20 f 3.86)/cm' at thefifteenth day. A-V shunt was positively correlated with the elevationof free portal pressure (FPP) (r=0.86, P<0.0l). Conclusion PVL-treated rats are optimal modal for the study of PHGSecond Part: Effect of disconnection and portacaval shunt onthe apoptosis in rats with portal hypertensive gastropathyObjective To evaluate the effects of disconnection and portacavalshunt on the apoptosis in rats with portal hypertensive gastropathy.Methods 42 wistar male rats were divided into 4 groups: (l ) normalcontrol group, (2) model control group; (3)disconnection group;(4)portacaval shunt group. The morPhosis of apoptotic cells wereobserved by Haematoxylin and eosin stain and the apoptotic indexswere measured by TUNEL method. Results Several apoptotic cellsscattered in tissue were seen in normal control group, more apoptoticcells seen in the other groups and the most seen in disconnectiongroup; The apototic index was increased significantly from (2.78i0. l7)% to (l4.46 f l .28)% after portal portal vein ligation (P < 0.0l ),then reached (l7.68 f 2.l3)% by disconnection, but decreasedsignificantly to (8.92 i 0.6l)% after protacaval shunt (P < 0.0l).ConcIusion In rats with portal hypertensive gastropathy, the numberof aPoptotic cells in gastric mucosa was increased significantly,which can be worsened by disconnection and corrected afterportacaval shunt.Third Part: Effect of disconnection and portacaval shunt on theexpression of caspase-3 in rats with portal hypertensive gastropathyObjective TO evaluate the effects of disconnection and portacavalshunt on the expression of caspase-3 in rats with portal hypertensivegastropathy. Methods 42 wistar male rats were divided into 4groups: (l ) normal control group; (2) model control group,(3)disconnection group; (4)portacaval shunt group. The expressionof caspase-3 mRNA was determined by reverse transcriptionpolymerase chain reaction method. Results The free portalpressure was increased significantly from (l. l5 f0. l3)kPa to (2.68i 0. l6) kPa after portal portal vein ligation (Pwt0.0l ), then reached(2.73 ?0.27) kPa by disconnection(P>0.05), but decreased significantly to (1.57?.23) kPa through protacaval shunt (P<0.0 1). So did the expression of caspase-3 mRNA, the expression of caspase-3 mRNA of normal control group ~. model control group disconnection group and portacaval shunt group were (0.48 ?0.02)~ (0.96?.04X (1.04?.06) and (0.65?.03) respectively. Conclusion In rats with portal hypertensive gastropathy , the expression of caspase-3 mRNA in gastric mucosal was increased significantly, which can be reach a higher degree by disconnection and decreased significantly after portacaval shunt.DUANJindong(general surgery)Directed by CHEN Yiren... |
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