| Early in 1920's, Growth hormone(GH) was known as one of pituitary hormones. The application of recombinant human growth hormone(rhGH) to clinic investigation appeared during 1980's. At present a lot of potential actions of therapy of rhGH have been discovered. Recent reports have indicated that rhGH has been used with a measure of success in patients with GH deficiency, including children and adults, and idiopathic dilated cardiomyopathy. When these patients were treated with GH, their cardiac performance, metabolism of myocardium, and clinic status improved dramatically, involving increase in the mass of myocardium and marked fall in the size of the left ventricular chamber. Recently, rhGH were used in our clinic practice to treat patients with atrophy of myocardium, dilation of ventricular chamber and cardiopulmonary deficiency in severe rheumatic cardial valvular disease, constrictive pericarditis and congenital heart disease. The results indicated that rhGH was benefit in the postoperative fatigue syndrome and clinic status improved. But there have been no data of ?--- investigation on the direct effect of Gil on the myocardium at home and abroad. The aim of this study is to investigate on these questions. This study is composed of two parts. The first part Objective To investigate the effect of OH on the heart myocytes, including morphology, electrophysiology and biochemistry of myocardium, and explore the mechanisms of the action of GH. Methods SD Neonatal rat myocardial cells were used as the material of the study and randomly divided into experimental and control groups. The hypertrophy myocardial cells model were established. Neonatal rat heart were minced and dispersed in PBS, and washed in pancreatin(370C, I 5mm) two times. Then the myocytes were rinsed with PBS and cultured in MEM culture medium supplemented with fetal calf serum for 2 ii, and later replated on cultural dishes. The concentration of rhGH in the culture 3 medium of the experimental group was O.5x10 and the control group was zero. The cultured cells of the two groups were divided into three parts: 1. LDH and CK levels of the supernatant liquid and the mixture of lysed cell by the Tris-HCI. were measured by AU-1000 automated, computer- controlled clinical chemistry analyzer. 2. The cultured cells were treated with anoxia condition (95% N2+5% C02) for 3 h, and then LDH and CK levels of the supernatant liquid of cultural medium were measured. 3. The proliferating amount of the active myocytes were measured with MTT method. The myocytes that was growing well were incubating in 0.2% ?6----- pancreatin at 37℃ for 2-3mm, and dispersed arid suspended in MEM culture medium supplemented with 10% fetal calf serum. 1 80u1 cell suspensions(the amount of cells was 5×105/ml) were put into each hole of the 96 holes .culture dish, and 20u1 rhGH added. The cells were cultured in 5%CO2 cultural chamber at 37℃ for 72h. Then MTT was added. Four to six hours later; the OD was measured by enzyme labeled device(QPSYS MR CB372), and a group 6 holes. Results 1. There was a significant difference in the morphology and...
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