Genetic And QTL Analysis Of The Content Of Vitamin E In Soybean

The Hefeng 25 variety with low seed VE content in Heilongjiang province and the Bayfield variety with high seed VE content in Canada were crossed.144 F2:7 recombinant inbred lines (RILs) were as materials. Theα,γ-andδ-tocopherol content and the total VE content were tested by HPLC in three places. The relativity were analysised between agronomic traits andα-,γ-andδ-tocopherolcontent and the total VE content of soybean in three places. The rules of inheritance of VE content (α-,γ-andδ-tocopherolcontent) in soybean powder were analysised by the major and multiple genes mixed genetic model. The genetic linkage mapping of soybean VE was constructed. The QTL were identified in theα-,γ-andδ-tocopherol content and the total VE content of soybean. And the QTL were identified in the agronomic traits in soybean. The interval of the QTL was fine QTL. The soybean variety was marker-assisted selected in the interval of the QTL was refined QTL. The results were as follows:1. Theα,γ-andδ-tocopherol content and the total VE content were tested in three places by HPLC. The method was simple and fit to test the VE content. Compare theα-,γ-andδ-tocopherol content and the total VE content in three places. The results were that a-tocopherol content and the total VE content in Haerbin were higher than those of the VE content in Hulan and Suihua.There were the relativity between the agronomic traits and protein, fat and theα-,γ-andδ-tocopherol content and the total VE content of soybean. It was significance that the VE variety was cultivated by screening the agronomic traits and protein, fat.2. Theα-tocopherol content was affected better than the y-and 8-tocopherol content by the environment by the genetic mechanism of vitamin E in the soybean.3. The QTL were identified in theα-,γ-andδ-tocopherol content and the total VE content of soybean seeds. The linkage group of the D1b was found in the three places. The linkage groups of the N, A2 and L were found in the two places.4. The fine QTL of soybean vitamin E was identified in the interval between the Sat239 and the Satt022 on the N linkage group. The polymorphism that was the primers was found in parents. It was valuable to divide the interval into little interval to the MAS.5. There were seven major QTLs about vitamin E in soybean. MAS related vitamin E in soybean was carried out in the intervals between the sat239 and satt022. Considering the all kinds of the agronomic traits, six strains which were good in the yield and quality about soybean VE were screened out. Their number was the 4,54,104,114,122,135.