| The interaction between drugs and biomembranes is the first step in the progress of various drugs playing their pharmacodynamics. Thus the research on both interaction is very important and necessary on understanding the efficacy and developing their biological properties. Ginsenosides molecules have become the hotspot topic because of their multi-pharmacutical activities and clinical functions. The study on the influence of ginsenosides on the lipids could provide experimental basises for discussing, realizing and resolving this problem. In this thesis, the differential scanning calorimetry techinique and Raman spectroscopy have been emploied the to study the interaction between five kinds of ginsenoside molecules (Rbl, Re, Rf, Rgl and Rh2) with different category phospholipid liposomes (DPPC, DMPC, DPPG and DPPE). Based on the thermodynamics and from spectroscopy view, the study on the interaction between ginsenoside molecules and biomembranes have been investigated in detailed for the first time on the molecular level. The conclusions are listed as follow:1. During the study on the interactions between five ginsenosides and DPPC, DMPC lipid bilayers respectively, the drug molecules have effects on the polar head group of phospholipids, however, this kind of interaction hasn't changed the conformation of O-C-C-N+ backbone in the choline group of PC bilayers. The polar head group is still extending parallel to the bilayer surface. Ginsenoside Rbl and Rh2 have significantly increased the fluidity of lipid bilayers. Ginsenoside Re, Rf and Rg1 have no such effects. Moreover, the ginsenosides have showed different degree on perturbating DPPC and DMPC lipisomes. For their different alkyl-chain length, the perturbations of ginsenosides are significantly stronger on DMPC than DPPC.2. In the study of five ginsenosides' separate affection on the DPPG and DPPE lipid bilayers, Rbl and Rh2 also distinctly decrease their phase-transfer temperature and increased the mobility of acyl chains. Ginsenoside Re, Rf and Rgl have little effects on the fluidity of DPPE and DPPG bilayers. On the difference of molecular structures, the protopanaxadiol ginsenosides have stronger effects on the acyl chains of DPPG and DPPE than the protopanaxtriol ones.3. From the gel phase to liquid crystalline phase transition, the four kinds of phospholipids have differences on the intermolecular attraction because of their different structure. Therefore, the perturbating degree of ginsenosides is different.4. As for the same lipid bilayer, there are various affections because of different ginsenoside molecular structures. This is related to the multi-pharmacutical activities of ginsenosides. |
