Study On Zymolytic Conditions And Application Effect Of Brewer's Spent Grains-Typed Bio-Organic Fertilizer

Bio-organic fertilizer is a kind of fertilizer made by organic residue which is fermented by microorganism with specific function, it has the same function of microbial fertilizer and organic fertilizer. Bio-organic fertilizer produces good effects to improving quality of soil and crop and increasing yield. brewer's spent grains, by-product of wine-brewing, can easily pollute environment. In this paper, brewer's spent grains were researched as zymolytic resource. Physical and chemical properties, degradation rate of carbon and nitrogen, effect of original zymolytic condition and effect of yield and quality of cowpea by using this fertilizer were systematically researched. It will provide a scientific basis for manufacture and application of brewer's spent grains-typed bio-organic fertilizer.The result shows that:1) Adding microbial inoculants can expedite decomposition, reduce weight of heap, enlarge water loss, lighten toxicity to seed, increase rate of carbon and nitrogen degradation rate, accelerate degradation rate of Cellulose, Hemicellulose, Lignin, reduce content of water-soluble carbon, water-soluble nitrogen, NH4+-N, accelerate Transfor-mation of NO3--N. The best inoculants was D3.2) Model was built by using the quadratic uniform-precision rotatable central composite design including four factors and five levels. Model showed that:The influence of pH value to loss of nitrogen was listed first, to degradation rate of cellulose and hemicellulose was listed forth, to degradation rate of total carbon and lignin was listed second. The influence of Inoculation fungal biomass degradation rate of total carbon and hemicelluloses was listed first, to loss of nitrogen was listed second, to degradation rate of cellulose and lignin was listed third. The influence of Ratios of straw to lee to degradation rate of cellulose and lignin was listed first, to loss of nitrogen and degradation rate of hemicelluloses was listed third, to degradation rate of total carbon was listed forth. The influence of water content to degradation rate of cellulose and hemicelluloses was listed second, to degradation rate of total carbon was listed third, to loss of nitrogen and degradation rate of lignin was listed forth. In this experiment, the suitable ranges of pH value, inoculation fungal biomass, water content and ratio of straw to lee are:6.40～7.02, 0.40～0.55%,64.7014～66.68% and 40.93～43.30%, respectively.3) Bio-organic fertilizer especially brewer's spent grains-typed bio-organic fertilizer can increase net photosynthetic rate in later stage of cowpea growth, improve the content of chlorophyll of function leaves, decrease transpiration rate, had little effect on stomatal conductance of function leaves. The contents of vitamin C and soluble sugar obviously increased, the contents of nitrate and nitrite decreased and the contents of crude fiber, protein nitrogen and non-protein nitrogen didn't have big effluence in edible parts of cowpea by Application of brewer's spent grains-typed bio-organic fertilizer.