Researches Antiseptic Mechanisms On The Bee Bread Of Xylocopa Tranquebarorum (Swederus)

In this paper, the anticorrosion mechanism of bee bread brewed by X.tranquebarorum was studied, the main research contents included nesting habit, the behavior of brewing bee bread, the changes of pH value and pollen activity during the process from corbicular pollen to bee bread, antibacterial activity of the raw extraction from bee bread, the source and function of 10-HDA in bee bread, function microorganisms and their metabolite extracted from bee bread and so on. The results layed a foundation for exploring new natural anticorrosion products. The main results were as follows.X. tranquebarorum which belonged to solitary pollinates and many flowers visiting insect had 1 generation per year in Nanjing, it nested in bamboo usually, but it was found nesting in reed occasionally. Peak period of brewing bee bread was in early May, so the best period for collecting bee bread was in early or middle May.The bee bread was composed of pollen and nectar mainly, which added by salivary gland secretion and microorganism from the body surface of female bee in the process of brewing. Because the 10-HDA from hypopharyngeal gland of female bees was added into bee bread, pollen germination was inhibited.68 microorganism strains were separated from bee bread of X. tranquebarorum, including 43 bacterium strains, 17 yeasts strains, 8 actinomycete stains, the above-mentioned 3 types of microorganism were also separated from body surface of female bee. Because of the existence of bacteria and yeast, the pH value of bee bread decreased gradually in the process of brewing bee bread, from 6.19 (P. lactiflora pollen) to 4.82 (3-day bee bread). Under the condition of such pH value, many bacterium and moulds could not grow normally.12 strains had stronger antibacterial activities among the above-mentioned 68 strains, actinomycete strain NF-34 had significant antibacterial activity to fungus and bacterium. According to its morphological and cultural characteristics, the physiological and biochemical properties and 16SrDNA sequence analysis, NF-34 strain was identified as Streptomyces sampsonii. The antibacterial metabolite of NF-34 strain had been identified asâ… Antibiotic of Water Insoluble by using Bioassay-guided Method.