| Eucalyptus dunnii is the cold-hardness and rapid-growth tree and could be properly cultivated according to the introducing in higher latitude of our country in late years. This species has been introduced in the higher latitude areas of the south areas of Hunan province, the middle and south areas of Jiangxi province, the north areas of Fujian province. The tree was introduced that has been reported in south sub-tropic of Lanning city of Guanxi Zhuan nation municipality in 80 ages of 20 century, till now the lone tree flowered and fruited that has been reported ,but has not been newsed about the group trees flowering and fruiting in china. The tree comparision to another Eucalyptus has long life history, when the tree flowers and fruits that must use more time to grow in nutrient period. The growth-period of nutrient of the tree overs 15years when the tree flowers and fruits in our land .The tree fruited and provided seeds that has not been reported in the introduction areas, for all that it has been introduced in many provinces from 80 ages of late century to now.The seed of Eucalyptus dunnii appling to afforestation is imported from the original area of Austria now and the seed of Eucalyptus dunnii is strict and expensive in market, the cost of the seed that it is more clare is highe and the price of average kilogram overs 70,000 yuan in international market. The seedling will be cultivated by 1 kilogram seed about 300,000-400,000 individual plant and invest the cost of seed expend between 0.15 to 0.20 yuan average Individual and increase the investion of afforestation of Eucalyptus dunnii. A great of money will be expended for buying the seed of Eucalyptus dunnii from Austria every year in china. The flower of Eucalyptus dunnii is pollinated through the pollen of even flowers, so the provenance of Eucalyptus dunnii is more confusion than anothers because of natural distribution in the original area of Austria. The growth of generation individual of Eucalyptus dunnii is various because of hybrid, the different individual of growth and cold-hardness will behave even diversity in planting in Minbei of Fujian province.The cold-hardness and rapid-growth characteristic of Eucalyptus dunnii is better ,it is difficult for clone of the tree to root, culture of seedling of clone is more difficult than anothers tree ,no matter what tissue culture or sticking difficultly provide the seedle for application in practic till this time. Although the success that rooting of clone of Eucalyptus dunnii has been reported ,the seedle of clone has not been used in production. A great deal of seed of Eucalyptus dunii is imported for culture of seedle every year ,but the seedle of clone of Eucalyptus dunnii is hardly used in practic. The results could not been getten by repeating experence that it had been reported for rooting of sticking and tissue cultivation. It is the main and difficult problem for providing the seedle of clone in production still, so the technology measure of clone of Eucalyptus dunnii is greatly obstacle in planting and popular. The obstacle limites planting and popular of elite individual of Eucalyptus dunnii and moving to more high altitude ,at the same time the potential of high output is limited in practic.The stands that had been afforestated by seedle of culture of seed of Eucalyptus dunnii in different years was investigated and it indicated that the individual growth of Eucalyptus dunnii exsisted more great differentiation in the growth of brand diameter, height, volume in stands, the elite comparision to averages exsists even varity in the growth of brand diameter, height and overs 100% and outdo to 100% more and more in the growth of volume in stands. the elite comparision to averages exceeds to 200% in the growth of volume of individual tree according to the selection and analysis, the best individual overs 400% than average in volume. The average output of stands in year is various low than anothers stands that was afforestated by clone seedles and the total facial of stands is not clear and the total output of stands is lower too ,because of the differentiation in the growth of stands is great. The growth result that was afforestaed by seedles of culture of seed of Eucalyptus dunnii is not perfect ,the potential growth could not be behaved and the volume quality was resulted to decreasing in stands at the same time. The technology and measures of clone of Eucalyptus dunnii was studied that is the potential cost of economy and sociality and grand magnificant in planting forward to higher altitude.Stusdying on the elite selection of Eucalyptus dunnii has been papered in higher altitude of middle-subtropic area, but the study of elite selection of Eucalyptus dunnii is only the primary period in china. The study of elite selection of Eucalyptus dunnii that the prehuman had done don't express the application cost in practic, because a great deal of seedles that are cultivated by the clone method has not still efficient technology measures in production and the hybrids of Eucalyptus dunnii is difficult in application because of long life history.The need of timber and timber organization are rapidly increased by sociality with the development of sociality and increasing of popular and brings up the great depression for environment that human exsists in. Eucalyptus has become the main special that is afforestated as the industry material stands in south areas of our country and ceaselessly planted forward to higher altitude of middle-subtropic area, because our country is poor in forestry ,in order to decrease the depression of need of timber and timber organization by the people's use, but many specials of Eucalyptus are of the poor characteristic of cold-hardness, they are limited to plant forward to higher altitude of middle-subtropic area in china. The characteristic of cold-hardness is the important selection factor of Eucalyptus dunnii for popularly planting forward to higher altitude of middle-subtropic area in china, but the elite adaptability of cold-hardness and rapid-growth was behaved during planting in the higher altitude of middle-subtropic areas in china .The elite selection of Eucalyptus dunnii mainly includes three aspects(the characteristic of rapid-growth, the characteristic of cold-hardness, the technology and measures of clone )and the technology and measures of clone is the key problem to settle down the selection aim, when the study of elite selection of Eucalyptus dunnii for popularly planting forward to higher altitude of middle-subtropic area in china is getten. It is the basic technology that preserves the elite characteristic of Eucalyptus dunnii and applies to the production. If the elite characteristic of selection of Eucalyptus dunnii is not efficiently behaved by the technology and measures of clone, the potiental cost could not be developed and used ,it will not settle down the antinomy that the needs of sociality for the timber and timber organization are discoveredThe growth rule of stands, individual growth, the adaptability of cold-hardness and the growth distribution of different individuals in stands are respectively studied on the introduced Eucalyptus dunnii in higher altitude of Minbei ,Jianyuang city of Fujiang province. The different growth rules that are systemicly approached in this paper and theory of recombine and mutation of individual generation of Eucalyptus dunnii hybrids were applied and the mutational individuals of Eucalyptus dunnii in the different ages stands are selected in introducing area. The mutational individuals of Eucalyptus dunnii in the different ages stands are investigated by the method that the sample numbers and area are respectively more over 500 ,4000m2 in investigation, the election criterion of elite trees was determined by applying"t"determination principle according to the normal distribution rule of stands growth and the cold-resistant and rapid-growth elite trees of Eucalyptus dunnii were elected based on the election criterion. The new selecting method that the theory of mutation of botanical and mathematics statistics were crossed and integrated was firstly advanced in this paper.The new method compares with"the 11 average standards tree and 5 dominant tree"that had been brought forward by prehuman is more strict and logic, the selection standards of elite individual could betterly be determined and select the elite according to the mutation of botanical, the new theory could avoid the errors that are formed by environment and selection standards that will be determined by human thought, the method was first proposed and named as the method of"t"determination principle, this method overed the problem that the election criteria is subjective long time in this paperThe clone technology of Eucalyptus dunnii is a magnificant technological problem that must be discovered for planting in the higher altitude of middle subtropic, but the success in sticking technology of Eucalyptus dunnii has been getten in sticking technology through experienment time and time, the efficient ingredient that has been getten could betterly induce the callus of the cutting of Eucalyptus dunnii and the rootings are differentiated from callus, at the same time ,the mechanism of rooting of Eucalyptus dunnii was discussed. The sticking technology of Eucalyptus dunnii can have succeedly been applied on culture seedles, at the same time the differentiate clone groups that the selecting standards is mainly determined as the characteristic of rapid-growth were constructed. The adaptability of cold-hardness could not be determined according to the fix quantify in preliminary selection because that The adaptability of cold-hardness is influenced by natural factors. the method of electrolyte leakage rate of determining the adaptability of cold-hardness had succedly been achieved by prehuman, the adaptability of cold-hardness of the different elite clone groups will be determined according to the fix quantify using the different elite clone groups and the method of electrolyte leakage rate. The mechanism of cold hardness had been studied on the characteristic of cold-hardness of plant by preman that they had achieved magnificant result from physio- biochemistry to gene, but the fix quantify study about adaptability of cold-hardness has no one to do in the different botanical in the different areas, the qualititive analysis was only done by prehuman. The adaptability of cold-hardness of the different botany in the different areas will be determined according to the method of fix quantify through using the method of electrolyte leakage rate and the maths model of extreme values distribution , the method is firstly advanced in this paper and applied to precialy determining the adaptability of cold-hardness of the different botanical in the different areas, the adaptability of cold-hardness was standardized by quantify, the method was named as the lowest temperature distribution of cold-hardness, at the same time the adaptability of cold-hardness can be divided into the poor adaptability of cold-hardness, normal adaptability of cold-hardness and the stronger adaptability of cold-hardness according to the method of fix quantify. The gene type of the cold-hardness and rapid-growth was repeatedly selected basing on the adaptability of cold-hardness of different elite clone groups of Eucalyptus dunnii that had been determined according to front method, the method could rapidly make decision and value the result before applying for the introducing trees that its are limited by temperature in introducing and guide the introducing in practice, the method was named as"the extreme-environment reflecting method". The decision was studied on the distribution of level and erect areas that the gene types of the cold-hardness and rapid-growth of Eucalyptus dunnii will be fitted on with the"the extreme-environment reflecting method"that have firstly been advanced by GUO Xiang-quan, HONG wei and so on. The evalution and decision making of the planting and popularing the gene types of the cold-hardness and rapid-growth of Eucalyptus dunnii could betterly be done basing on front theory,also"the extreme-environment reflecting method"will provide the system theory and reference in order to decrease the venture of investion in practic. The growth rules of adaptability, clone, the elite selection, the gene types of the cold-hardness and rapid-growth of Eucalyptus dunnii and theory of forestry breeding and so on were probed for the introduction and planting in the more northern areas of middle subtropic and the three new application theories was first proposed in forestry culture and overed the application of forestry culture in this paper, it would provide guidance in the forestry culture theory of study and using, the main fruit includes these aspects:[1] The growth rule of young trees during year of Eucalyptus dunnii was observed on fix interval-time in the introducing area of Jianyuang city Fujian privince, the results indicated that the tree begins to sprout when it overs 10℃.The tree firstly begins to sprout on the initial stages of March in general and the growth of height of young trees during year of Eucalyptus dunnii is slower during March to April and is more speedy on M ay and enter the first fastigium from the late of May to August, the growth quantity of height emerges decreasing during September and enter the second fastigium from Octember to November and behaves rapidly decreasing from the late of November. Eucalyptus dunnii belongs to the growth type of total year and doesn't form the up-bud in winter. The adaptability of the cold-hardness of young trees during year of Eucalyptus dunni is different when it is stressed in winter because of the individual differentiation and include three degrees. The poor adaptability of the cold-hardness behaves the winter kill of up-bud and the growth of height stops. The normal adaptability of the cold-hardness behaves that color of leaf become red and the young leaf and up-bud are freezen. The stronger adaptability of the cold-hardness behaves that leaf keeps green and slowly grows still. The growth rule of young trees groud-diameter during year of Eucalyptus dunnii is basic the same as the height's, but the growth quantity of ground-diameter is higher in the second fastigium than the first fastigium, the main growth quantity of ground-diameter emerges in the second fastigium for the young tree during year. The average growth quantity of height of young trees during year of Eucalyptus dunnii is about 3.0m and The average growth quantity of ground-diameter is about 3.0cm.[2] The growth of stands of Eucalyptus dunnii in the second years emerges great difference with the seedle that was cultivated by seed in the introducing area, the individual growth differentiation of height and brand-diameter is obvious. The individual growth of height respectively distributed in 2.7-10.4m,2.9-10.8m,the distribution inter-distance is respectively 8.7m,7.9m and The individual growth of brand-diameter respectively distributed in2.8-10.6cm,2.5-10.9cm,the distribution inter-distance is respectively 7.8cm,8.4cm in the investigation stands. The investigating resuilt in the stands of 2 ages is analysized that it indicated the individual distribution of height and brand-diameter in the stands is normal distribution, the stands of 2 ages that has well survival ratio could cover in general and begins the growth of volume accumulation. The individual growth differentiation of volume is the same as the height and brand-diameter and the individual distribution of individual growth of volume is normal distribution in the same interval in stands. The earlier differentiation of individual growth characteristic behaves the dominance of individual inherit and it is profit for selection of elite characteristic, but The earlier differentiation of individual growth characteristic of height and brand-diameter will influence the total growing quantity of stands and quality of timber.[3] The growth rule of stands of Eucalyptus dunnii in the third years is the same as the stands of Eucalyptus dunnii in the second years, but the individual differentiation is more great comparision the third years to the second years. The stands of Eucalyptus dunnii of 3 ages enters into the important period of volume growth for the management aims of short-periods of Eucalyptus dunnii and the annual growth quantify of individual volume increases and the differentiation of individual volume growth becomes more obvious because of the differentiation of the growth of brand-diameter and height. The individual growth of height distributed in 4.2-16.5m,the distribution inter-distance is 11.3m and the individual growth of brand-diameter distributed in 3.4-15.2cm, the distribution inter-distance is 11.8cm and the individual growth of volume distributed in 0.0025-0.1213m3, the distribution inter-distance is 0.1183m3and the numbers proportion of the individual growth of volume distribution in 0.07-0.13m3 is 1.1% in the investigation stands. The growth ratio of height to brand-diameter is obviously changing during the individual growth of stands. The total growth of stands volume is the aim of production, so the elite characteristic of individual volume growth that was selected as the aim of annual volume growth quantify basing on the differentiation of individual volume growth will provide much more profit for production management. The individual growth of volume comes from the exponential product of brand-diameter and height and the individual growth index of volume may be regarded as the selection index of brand-diameter and height when the selection index of brand-diameter and height couldn't be satisfied in order to satisfy the needs of elite selection.[4] The mutative individual of stands was selected in the earlier period with the great sample method basing on the earlier differentiation of characteristic of Eucalyptus dunnii during the different period in the different stands and the normal distribution of growth of brand-diameter,height and volume in stands. The great sample method is the sample number overs 500 individual and the area that is investigated overs 5000m2,when the mutative individuals are selected in investigation. The mutative individuals of Eucalyptus dunnii are selected with the three index factors that includes brand-diameter ,height and volume and the selection standard is determined with"t determined rule"of normal distribution in the different stands in the introducing area. The selection method is advanced in first in this paper that could be applied to the elite selection and is more impersonal and adaptive than"the 11 average standards tree and 5 dominant tree"that had been brought forward by prehuman. The method that the selection standard is determined with"t determined rule"of normal distribution bases on the theory of mathematics statistics and gene reform,it is different from anothers that had been brought forward by prehuman and it is strict maths logic and overed the problem that the selection standard is subjective in the elite trees election long time. using the method ,6 individuals of 1 age,5 individual of 2 ages ,2 individual of 3 ages ,2 individual of 4ages that the height- growth are respectively over 5.76m,8.8m,15.3m,16.59m and the brand-diameter growth are respectively over 8.93cm,14.12cm,15.63cm from 2 to 4 ages stands were elected ,these growth were obvious large than averages of the different stands.[5] The clone of Eucalyptus dunnii is the important and technologic problem to discover planting forward to higher altitude area of middle subtropic. The clone rooting of sticking of Eucalyptus dunnii belongs to this type that callus differentiates unfix-root. using orthogonality design and additive1,additive2, NAA,6-BA,vitamin,time were regarded as treating factors that its were divided into three degrees,the parenchymatous meri-tissue is induced with the botanical growth regulator and callus was formed in cutting of stick. The efficient combination that it was gained using these factors which are 2 degrees of additive1 and additive2, 120ppm of NAA,40ppm of 6-BA,20 ppm of vitamin,1.5h of time can betterly induce callus in cutting of stick and differentiating unfix-root by time and time again. Applicating cofront mothed could induce callus and ratio of calluses is respective much more 80%,20-60% for cutting of stick of 1 age ,2 ages, ratio of calluses is lower 15% for cutting of stick of 3 ages and overs and many stick calluses could not be induced and unfix-root could not be differentiative. The mother-trees must be cutted off a little of branch in order to advance germinating new branch for the over 2 ages mother-trees in beforehand. experiment indicated that the differentiating ratio of unfix-root could not over 30%,but the fruit can be repeated and the differentiating ratio of unfix-root will be advanced in future ,in the same time, the temperature adapts in 25-30℃,humidity adapts in 80%,soil adapts in middle. using confront method, callus can be observed in 7 days under the adapted condition.[6] The adaptability of cold-hardness is the important and strict factor for Eucalyptus dunnii moving forward to the higher altitude area of middle subtropic. The adaptability of cold-hardness of the different elite selected of Eucalyptus dunnii is various because of the differentiation of growth phenotype. The self-mortality temperature of the different individuals of Eucalyptus dunnii elite and average could be determined with the refrigeration plant of manual control, after the different clone groups of Eucalyptus dunnii elite and average are stressed by lower temperature for simulating natural condition according to-1℃,-4℃,-7℃,-10℃,-13℃. The daily changing of the different individuals of Eucalyptus dunnii elite and average were determined respectively in 8:00,10:00,12:00,14:00,16:00 in normal condition, and the connection of The daily changing of electro-conductivity with self-mortality temperature of the different individuals of Eucalyptus dunnii elite and average was probed The adaptability of cold-hardness of the different clone groups of Eucalyptus dunnii elite and average in the introducing area could be determined in quantify using the maths model of extreme values distribution in the introducing area and the different clone groups of Eucalyptus dunnii elite. The different adaptability of cold-hardness of plant that is quantified in the different area is firstly advanced in this paper and the degree of adaptability of cold-hardness of the different botany in the different areas are divided into three degrees in quantify and respectively named the poor,normal and stronger adaptability, this method discovers the problem that the different adaptability of cold-hardness of plant in different area is narrated in faintness and unquantify method for long time, making the decision of introducing with the adaptability of cold-hardness of plant in unquantify generally results to natural calamity bringing up the lower temperature streession, but this method could betterly make the decision and evaluation and the cost is higher in production. using this mothed, the different adaptability of cold-hardness of the different elites were re-elected . The resuilt indicated that jingshan-02, jingshan-03 are of the strongest adaptability of cold-hardness when they are planted up to altitude 600m, jingshan-04, jingshan-05 jingshan-06, jingshan-09 jingshan-11, jingshan-12 jingshan-15are of the normal adaptability of cold-hardness, but jingshan-01, jingshan-07, jingshan-08, jingshan-10 jingshan-13, jingshan-14 can't be adapted to popularize and jingshan-02, jingshan-03 will adapt to culture the clone[7] The physiological reflecting for the different adaptability of cold-hardness of plant individual behaves obviously vary during the lower temperature stress. The physio-biochemistry index of action of SOD,MDA , index of un-saturation fatty acid of the different elites of Eucalyptus dunni and average is determined after the different elites of Eucalyptus dunni and average are stressed by lower temperature for simulating natural condition according to-1℃,-4℃,-7℃,-10℃,-13℃. determining of physio-biochemistry reflecting results indicated that the physio-biochemistry index of Eucalyptus dunnii elite exsisted obviously vary. the resuilts that the physio-biochemistry index had been determined is similaritive to self-mortality temperature index of the different elites of Eucalyptus dunnii .The physio-biochemistry index of plant may betterly reflect to the adaptability of cold-hardness of plant individual. The physio-biochemistry index of plant is important index and determining standard during selecting elite individual. but it can't exactly determine the critical lower temperature of botany individual and can exactly reflect changing of physio-biochemistry of botany individual, the index only indicates the adaptability of cold-hardness of plant individual in subjectivity[8] The introducing of plant is limited by many factors of geography and it is different for the different plant and the different areas to introduce planting. The limited factor for Eucalyptus dunnii to move planting in the higher altitude of middle- subtropic is the extreme lowest temperature of introduce area, the distribution of the extreme lowest temperature of introduce area determines the possibility of introduce. The"the extreme-environment reflecting method"that have firstly been advanced by GUO Xiang-quan, HONG wei and so on could betterly make the decision and evaluation before introducing of plant, the decision that the different clone groups of Eucalyptus dunnii elite is planted in the level and erect fitness area is made and evaluates the fitness planting area for the different clone groups of Eucalyptus dunnii elite with the records of the extreme lowest temperature of Jianyang city of Fujiang province and the self-mortality temperature of the different clone groups of Eucalyptus dunnii elite ,this method overs the failure and damnify that evaluation was made by the historic method .
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