The Preliminary Research Of Plasma Protein Involved In Bovine Mastitis

The mastitis is the inflammation of cow mammary gland, its occurrence is a combined action result including the pathogenic microorganism, the environmental factor, the management factor, the cow own and the hereditary factors and so on. The mastitis is one of diseases that causes heavy economic loss and influences the development of dairy industry in the worldwide.It has became the hotspot not only in the fieldof livestock and veterinarian but also in food security. Following the development of biotechnology, more new technologies are applied to investigate the mechanism of mastitis pathogenesis. In this study, plasma proteomics analysis was performed for analysis the protein differentiations between healthy and patient cows, as well as around the calving period. It will be help for detecting mastitis process on pathological levels, and seeking for the proteins related to mastitis to provide the reference for mastitis treatment ahead of time. The main results are as follows:First, different methods for the preparation of plasma sample were compared—DEAE Affi-Gel blue column, anion exchange column and ultrafiltration tube. More steps were needed, and the enrichment effect was not better. The heating with 10%SDS and 2.3% DTT to denature sample at 95℃for 5min, IEF on pH 4-7 IPG strips were better for effectively improving the resolution and intensity of low-abundant proteins.Second, analysis of different proteins between health and mastitis cow. Plasma was collected from health and mastitis cow respectively. According to somatic cell counts (SCC), plasma from mastitis cow was divided into two group: mastitis1 (2000x103cells/ml>SCC>400 x103cells/ml), mastitis2 (2000x103cells/ml