Interactions Of Insect Pests Through Rice And Acquirement Of The Mutant Of Rice-Related Gene OsRLK

After being fed by phytophagous insects, the damaged plants can produce and release the volatile compounds which are different qualitatively or/and quantatively from the healthy plants, and the compounds can affect the phytophagous insects themselves and others. In addition, plant cells can feel the stimulation of the biotic and abiotic factors through receptor-like protein kinase and conduct the signal from extracellular to intracellular through the cellar membrane, which can activate the defense genes of intracellular downstream and make the plants get the defense function. The main results as following:1.Using the dynamic techniques for headspace desorption, the rice volatiles from the healthy plants and damaged plants were trapped after damaged by Chilo suppressalis and Cnaphalocrocis medinalis for 24h. The results showed that 28 kinds of volatiles compounds were trapped from the damaged and healthy seedlings, and 16 kinds of volatile compounds were qualitatively determined including 9 terpenoid, 4 alkanes, 1 ketones, 1 alcohols and 1 esters. 22 volatiles were trapped from the healthy seedlings, and 2-heptanone,α-pinene, (+)-limonene, linalool,α-copaene, n-tetradecane, zingiberene, (-)-α-cedrene, (+)-cedrol, n-heptadecane, n-octadecane and n-nonadecane were identified. 26 volatiles were trapped from the rice damaged by C. suppressalisr larvae, and terpenoidα-pinene, (+)-limonene,α-copaene, (-)-α-cedrene, (E)-linalool oxide, linalool, (+)-cedrol,zingiberene, 2-heptanone, 2-heptanonol, n-heptadecane, n-octadecane, n-nonadecane and n-tetradecane were identificated. 26 volatiles were trapped from the seedlings damaged by C. medinalis larvae, and 9 terpenoid, includingα-pinene, (+)-limonene,α-copaene,β-caryophyllene, (-)-α-cedrene, (E)-linalool oxide, linalool, (+)-cedrol and zingiberene, were identificated, and the others (2-heptanone, 2-heptanonol, MeSA, n-heptadecane, n-octadecane, n-nonadecane and n-tetradecane) were also identificated.2.Using EAG technology, the response of C. suppressalis male adults, and C. medinalis male and female adults to the rice volatiles from the rice seedlings was tested. The results showed that Except n-tetradecane, the response value of C. suppressalis male adult to 2-heptanonol, n-octadecane, n-nonadecane, limonene, linalool and 2-heptanone increased with increasing sample concentration. The threshold of C. suppressalis male adult to seven substance above is 1ng/μl. Except n-octadecane and n-nonadecane, the response value of C. medinalis female adult to 2-heptanonol, n-tetradecane, limonene, linalool and 2-heptanone increased with increasing sample concentration. Except n-octadecane and limonene, the response value of C. medinalis male adult to 2-heptanonol, n-tetradecane, linalool and 2-heptanone increased with increasing sample concentration. Comparing the response value of EAG between C. medinalis female adult and male adult, it was found that the EAG response value of linalool and n-octadecane to the female and male adult had no obvious difference, and 2-heptanonol, n-tetradecane,2-heptanone and limonene cause the EAG response value of female adult significantly greater than that of male adult. Especially the dose of limonene concentration, when the limonene concentration was lower than 0.001ng/μl, it had great influence on male adult. But when limonene concentration was higher than 0.001ng/μl, it had great influence on female adult.3.Field investigation showed that the number of Laodelphax striatellus on the seedlings damaged by C. suppressalis was much more than that on the healthy seedlings. The growth period of L. striatellus nymph on the seedlings damaged by C. suppressalis was much shorter than that on the healthy seedlings, and the life of female adults was further longer than that of male adults, but the wing dimorphism, sex ratio and preoviposition period had no difference whereas the fecundity of L. striatellus on the damaged seedlings was much more than that on the healthy seedlings. L. striatellus nymphs on the damaged plants preferred to the stem of feeding sites of C. suppressalis. L. striatellus female adults prefered to the damaged plants. 17 free amino acids in the plants damaged by C. suppressalis and healthy plants were detected. Through the comparison of the plants damaged by C. suppressalis and the healthy plants, threonine content in damaged plants decreased somewhat, and the contents of aspartate, serine, glycine, isoleucine, leucine and phenylalanine in damaged plants significantly increased whereas the contents of glutamate, alanine, cysteine, valine, methionine, tyrosine, lysine, histidine, arginine and proline did not significantly change.4.Using RT-PCR, the OsRLK(receptor-like protein kinase)gene from the rice fed by C. suppressalis was isolated, and sequencing and comparative analysis of Blastn and Blastp confirmed that the cloned gene was OsRLK. By a series of connection and transformation after connecting the OsRLK with expression vector, the over expression vector was obtained. The OsRLK was transferred to rice successfully using agrobacterium-mediated transformation and the over expression of transgenic plant including the OsRLK was obtained.