Effects Of Immunosuppressive Virus Infection On Interferon-gamma Production In The Splenocytes Of SPF Chickens

Many viruses are likely to cause immunosuppression in chickens, such as Marek's disease virus (MDV), reticuloendotheliosis virus (REV) subgroup J avian leucosis virus (ALV-J), chicken anemia virus (CAV), reovirus (ARV), Newcastle disease virus (NDV), infectious bursal disease virus (IBDV) and etc. Epidemiological investigation indicated that immunosuppressive viral infections are very common in chickens. Moreover, immunosuppression could become more serious when dual or multiple viral infections and their interactions happened. Immunosuppressive diseases have brought our poultry industry great endangerment.However, the mechanism of virus-induced immunosuppression has been unknown to us, especially the role of cytokines in avian immunosuppressive viral infection, pathogenesis and immune response. Many reports demonstrated immunosuppression induced by some pathogen were related to the secreting levels and abnormal expression of cytokines. Most researchers measured interferon-gamma(IFN-γ) mRNA by reverse transcript-polymerase chain reaction(RT-PCR)and only showed the stimulation to IFN-γsecreting from splenic leukocyte by viral infection indirectly. In this study, double-antibody sandwich enzyme linked immunosorbent assay(ELISA) was established based on commercial chicken IFN-γmonoclone antibody(mAb) and IFN-γstandard, and directly quantitative measured the levels of IFN-γfrom splenic leukocyte of specific pathogen-free chickens infected by REV, MDV, CAV and ARV. Spleens were collected, Splenic leukocytes were prepared and cultured at various times after infection with REV, MDV, CAV and ARV and compare them with those in uninfected age-matched controls, so as to delineate the potential role of IFN-γin the mechanism of virus-induced immunosuppression.Chicken IFN-γwas measured by biotin-avidin-amplified double-antibody sandwich ELISA...