OsWRKY10 Gene Function Research Via RNA Interference And Endurance Analysis Of Ferritin Transgenic Rice Plants To Iron Stress

Rice DNA-insertion mutant Osdg grows more slowly. Target gene is a WRKY transcription factor, named as OsWRKY 10. Contrasted to Oryza sativa L. subsp. japonica. , Osdg has longer development period, shorter plant, decreased tillering number, shorter spike length, lightened mass of 1000 grains, slowered germinating speed. Namely, Osdg presents obvious mutation characters. However, some researches show that DNA-insertion lines of transcription factors seldom exhibit altered phenotypes. So, to clarify matters, cDNA segment of target gene is used to make a special dsRNA interference vector. Oryza sativa L. subsp. japonica. is transformed to get silencing lines. Finally, 56 transgenic plants are obtained. As observation results showing, except for 14 transgenic plants, the rest show mutation phenotypes more or less, and 17 interference plants exhibit obvious mutation characters. These silencing lines have longer development period, shorter plant, decreased tillering number, shorter spike length. That is to say, they recur the mutation characters same to Osdg. All these tell us, OsWRKYlO closure results in the mutation of Osdg. This gene not only controls the development period of rice, but also participates in a series of processes such as seed germinating, plant growth, jointing, tillering, development of spike and grain, seed filling, etc. In a word, OsWRKYlO widely controls growth and development of rice.Transgenic rice plants with ferritin overexpression have been researched with iron stress condition. Although, all the plants tested in iron stress have showed shorter plant, decreased chlorophyll content, increased MP. But, when compared with transgenic plants, the CK is much more influenced by the iron stress. All these tell us, the transgenic plants have stronger endurance to high Fe²⁺ concentration. However, unexpected, although all plants of stress group have largely increased iron content in their leaves, but there is no distinct difference between transgenic plants and CK. Thus it can be seen, there is no difference at iron absorption capability between different plants series during high Fe²⁺ stress. Hereby, we think, whether a plant show iron stress endurance lies on the iron storage form in it. Furthermore, iron content in plant is influenced by environment iron concentration, therefore, whether it is absolutely feasible to increase iron content in plant via ferritin overexpression need more research.