Genetics Analysis And QTL Mapping Of Glutenin And Starch Swelling Properties In Common Wheat

A population of 175 recombinant inbred lines (RIL) derived from common wheat Jing 771 and Pm97034 was planted in Beijing and Xinjiang location. The main objectives were to investigate the separation and distribution of wheat quality traits in RIL population, expound the interaction effects of genetic and environment on quality traits, reveal the dynamic changes of GMP content, and map several quality traits QTL loci on the wheat chromosomes.Major results are described below:1. There was a complex correlationship among wheat quality traits. It was significant positive correlation among swelling index of glutenin (SIG) value, Zeleny sedimentation value(Zeleny-SV), GMP content, swelling power(SP)and gluten index(GI). No correlation was found between SIG value and dry glutenin content(DGC), and between SIG and wet glutenin content(WGC). While, a significant negative correlation was found between SIG value and alkaline water retentiveness capacity(AWRC). AWRC was significant positive correlation with WGC and DGC. Flour whiteness was positive correlation with SIG value, Zeleny-SV, DGC, WGC and GI, significantly. But negative correlation with AWRC, and no correlation with GMP content and SP.2. The dynamic changes of GMP content indicated that GMP accumulated at low level in early stage of grain filling, then ascended gradually, but descended about 22 days after fertilization, then ascended continually up to maturity. The results of conditional genetic variance components showed that the dynamic changes of the GMP net genetic effects were similar to GMP content accumulation.3. The effects of HMW subunits combinations of different allelic loci and different subunits at the same loci on quality traits were analyzed quantitatively in Beijing and Xinjiang location. The results showed that (1,17+18,5+10), (1,14+15,5+10) were the best HMW subunits combinations for improving the Zeleny-SV, SIG value and GMP content. (N, 14+15, 5+10) was better for improving the AWRC and SP than other subunits combinations. The influence of subunits combinations on improving the flour whiteness and declining the flour yellowness were different in different location. For example, (1,17+18,2+12) has a positive effect in Beijing, but(N,17+18,5+10) was best in Xinjinag. The effects of HMW subunits encoded by the same allelic locus on quality traits were also different. 17+18 subunit combination was the first in comprehensive rank on the effects of wheat quality traits measured, then 14+15 subunit combination, and then 5+10 subunit combination was ranked the third. It's more important of Glu-B1 loci for wheat quality improving.4. 129 SSR markers were mapped on 21 chromosomes by Mapmaker3.0. Length of linkage map was 2106.2 cM, with an average distance of markers was 16.32 cM.5. QTL analysis by combined comparison and epistatic test in different environments showed:5.1 Additive effects was main genetic behavior for most quality traits, and addition-addition epistatic interaction was found between different allelic locus commonly. There was the common QTL in traits with high correlation coeficients.5.2 Four QTLs located on 1AS,1DL,2DS and 5BL were associated with Zeieny sedimentation value; the main effects QTL of SIG value located on IAS, 2AL, 2DS and 5BL; and there were three QTLs influenced GMP content on the 2DS,3BS and 7BL respectively. Two QTLs on 3AS and 4A near the cen were associated with AWRC, and two QTLs on 4B and 7DS influenced flour swelling power.5.3 Two QTLs located on 1DL and 5BL were associated with WGC, the "QWgc-1D" was a QTL controlled by genetic and environment meanwhile. It was different from other quality traits, as its interactive contribution rates were higher than additive contribution rates. Three QTLs were mapped on 1DL,2DS and 5BL for glutenin index.5.4 No QTL was found associated with flour whiteness by combined comparison, but one QTL was found near the cen of 3A chromosome by epistatic test, and addition-addition epistatic interaction with 3B was tested. One major QTL located on 7AL was associated with grain yellow pigmen...