Study On Molecular Ecology Of Camptotheca Acuminate In Response To Heat Shock

Camptotheca acuminata is a tree unique to China, producing terpenoidindole alkaloid (TIA) 10-Hydroxycamptothecin (HCPT) and camptothecin (CPT), which possess strong anti-cancer bioactivity. The molecular ecology of heat shock to Camptotheca acuminate seedlings with the theory and method of functional proteomics was systematically studied. By the study we attempted to elucidate the molecular mechanism of ecological adaptation and evolving of plant to heat shock in the method of physiological ecology, molecular biology of plants.The research measured the photosynthetic potential changes when the Camptotheca acuminata seedlings were treated by heat shock, at the same time the physiologic index dynamic changes were measured as follows, they were MDA, chlorophyll and so on. We also measured the metabolized changes about the two important alkaloids of Camptotheca acuminata. Under the different treated conditions, differential bands were obtained through the denatured gel electrophoresis, then mainly according to the 2-D Electrophoresis, the dots appeared by the coomassie brilliant blue dying, thus 11 dots were separated. Of these dots, 6 including 7 kinds of proteins were primarily identified in term of the peptide quality fingerprinting map. Then, 5 kinds of the 7 proteins were diagnosed on their ecological acclimation function during the heat shock response by the biological informatics.The study discussed the molecular mechanism on heat shock metabolism regulation of camptothecins. On this base, combined with the protein data, the ecological acclimation of Camptotheca acuminata to heat shock response was analyzed. Then we concluded the molecular ecological mechanism of Camptotheca acuminata to heat shock response. Simultaneously, we used the Camptotheca acuminata heat stress as mode, probed into the possible mechanism on Camptotheca acuminata drought resistance modulated by camptothecinmetabolism.