The RAPD Analysis Of Changshan-huyou And The Research On The Varying Regularities Of Its Fruit Quality Together With The Processing Technology And Utilization Of Its Fruit

Changshan-huyou is a new species of citrus native to Changshan County, Zhejiang Province. Since its discovery at the end of 1970s, it has won several prizes in different national appraisals. As the first top-quality hybrid citrus in China, national green food and brand-name and quality fruit, Changshan-huyou is a rare major developed top-quality high-efficient and high production economic tree species. Hence, it is necessary to make in-depth and all-round systematic researches on Changshan-huyou. The researches are of vital realistic significance and distinct social and economic efficiency. There is no report on such researches abroad, so these series of researches will even be of very important academic value.This paper makes a molecular biological study on Changshan-huyou on the basis of summarizing the progress of researches on it. After repeated experiments, through adopting the improved CTAB method, we succeed in extracting high-density and purity DNA from the leaf of Changshan-huyou. The density averages 272.86#g ?mL-1, and about 82#g DNA can be obtained from per gram's fresh leaf. The purity reaches 1.56 on average. Both of the density and purity fully meet the demands of RAPD analysis and other molecular biological analysis. The paper also makes RAPD analysis of 12 popularized elite clones and 6 other fine plants. It proves that the RAPD response system determined in the experiment fully fits the cloning procedure(condition); 103 random cloning sites are achieved from 12 random chosen primers. On average, every primer creates 8.6 identifying sites, yet no polymorphic site is discovered. It infers from the analysis that there is no molecular difference between the 12 chosen elite clones of Changshan-huyou and the 6 other fine plants . The economic quality difference is mainly influenced by such factors as the phase of growth, the condition of ecological environment and the level of management.At the experiment site in Changshan county of Zhejiang province, standard trees were selected in 2000 and 2001. The fruits at different orientation were examined and analyzed. The result shows that the size of fruit increases in shaped form with the prolongation of growth. There are two rapid growth periods in a year: the early part of July and the mid September. The weight increases likewise but it alternates with the increase of fruit size. The change of the vertical diameter and the horizontal diameter are very similar except that the latter increases obviously faster than the vertical diameter does, especially in early August. The orientation has effect on the growth to some extent. It is showed by the standard deviation analysis that the vertical and the horizontal diameter have significant difference in different orientations and growth periods. The index of fruit and the change of the weight of fruits have no significant difference in various orientations, while they have in different growth periods. The fruits of Changshan-huyou have very similar index as 0.87 when they are ripe, which shows that they can not be classified by index since there is no obvious difference in the shape of fruits.By means of routine analysis, the quality and the variation during the storage of fruits of 3 different types of Changshan-huyou and 12 elite clones are analyzed and counted. The findings show that the weight of various types and clones of Changshan-huyou decrease in the course of storage, while the edible ratio increases continuously. The water content in all parts is on decrease, of which the greatest decrease is in that of the pericarp. The decrease in the white seedcase and thecapsular cover follow next. But the water content in sarcocarp changes very little and keeps at the high level of 87-92%. Therefore, there is an evidence that the fruit of Changshan-huyou has a strong capability of retain freshness. The acid content decreases and the content of soluble solid fluctuate greatly. The content of total sugar and reducing sugar are similar in change, appearing an up-down- up trend. The ratios...