Studies On Uterine Involution In The Ewes

The changes in ultrastrUctllTC of uterine tissues, degradation of co1lagen and myosin,isolation and identification of microbial species in the uterine 1umen, ultrasonic image of theuterus and cellar aPoptosis in the uterine tissues in the aduIt ewes (n=8) were studied by usingtransmission and scan electron microscope, amino acid ana1yzer, B-mode u1trasound scanner,cultUres of uterine microbial species during the period of uterine involution. The objectives ofthe study were to make clear the cellulas subcellular, molecular and dynamic changes of theinvoluting uterus in the POstPartUm ewes, the re1ationship betWeen uterine degrading andaPoptosis, the suPersonic changes of the involuting uterine, changes of HrrRO and 3MHlS inperiphera1 blood and urine and their relationship with uterus involution, the relationshipbetween microbial flora and uterine involution. The results showed that:l. From the view of the partUrition records, ewes in Heilongiiang kept their good traitsof heat and labor without seasonality. They can be finished all round the year. And thenumbers of partUritions were higher in winter and spring than in summer and autumn with themean 1itters of 2.35 per liberaion. The time betWeen parturition and the next heat periodranged from 36d to 85d.2. From the view of physiological indexes measured in peripanrent ewes, ewes showedhigher temperatUre, slightly lower number of respirations and higher number of pulses in theday 3 prepanrition. Body temperamre, both the number of respirations and pulses increasedon the day of partUrition. Wthin the 5 days postPartUrition, body temperatUre and the numberof pulses decreased slighly, however, the number of respirations continued to increase; on theday 12 postPartUrition, body temperatUre increased significanly, but the number ofrespirations and pulses decreased to the norma1 level. The number of hemoglobin (Hbs) andRed blood cells mpC) are lower than the control grOup during the day 3 prepartUrition andthe dny 5 postptherition, suggesting that ewes were in the anemic state during the peripamrentperied. The changes in the coatent of neutral White cells, which was in consiStent with those oftemperature, reflect the state of local inflammation of the ewes.3. Ewes fmished nierus involution in 25 days postPartUrition, morphologically. Thevolume of uterus, lochia and the diameter of caruncIe in the ewes decreased during thepostPartUrellt period. Ewes finished lochia discharge at day 10 postPartUIn, the thicknes5 ofuterus wall increased within the day l-7 postPta, then decreased there after. The cervixbecomes loose at the first day postPrtm, and increased tO the thickest at day 7 postPta,the cervix finished involution at l9 days postPartUm.4. The resultS from the LM and EM examination showed that the endometriaI wasdamaged pedially due to the partUrition, the cells around the damaged area breaken down,putrescence and shed; the new ePithelial cells aPpeared at l3 dnys postPartUm around the32002^5^damaged areas, then covered the whole endometrial at 25 days postpartum, and finished recovery at 3 1 days postpartum. The psedostratum column epidermis finished growth at 25 days postpartum, however endometum recoveried completely at 3 1 days postpartum.5. The degradation of collagen in uterus was one of the causes of the reduction of uterus volume postpartum. There were many cells including macrophages, plasma cells, fibroblasts, lymphocyte, which took part in and were helpfull for the involution.6. The apoptotic cells existed in the uterine tissues during the uterine involution, and were one of the ways reducing the uterus volume. Many cells started to die by apoptosis when the acute regeneration occurred in the uterine tissues in 19 days postpartum. There was no significant difference between the results from the methods of in-situ end dye and HE coloration.7. The results from the B-mode ultrasound scanning showed that the diameter of the uterus decreased, an...