Studies On The Pharmacokinetics And Pharmacodynamics Of Danofloxacin In Healthy And MG-E.Coil Infected Chickens

In this paper, the vitro activity of danofloxacin against MGs~ and E. Coil. 078 were determined by tube-test serial two fold dilution; plasma pharmacokinetic test bioavailability tissue kinetics of danofloxacin in 240 healthy and M6-E. Coil infected chickens were investigated by using the HPLC method: and therapeutic efficacy of danofioxacin on 210 chickens infected with MG-E.Coil. were studies following oral administration through drinking water, The concentrations versus time data were analyzed using MCPKP pharmacokintic programs0 The dynamic interrelated analysis of danofloxacin levels between plasma and tissues were performed with pharmacokintic method and regression analysis method0 The indirectly predictive equations about the drug concentration in tissue were established,, The results of in vitro activity of danofloxacin indicated that MGs6 and E. Coil.078 were high sensitive to danofloxacin and the MICs were 0.063 3.?g/ml, The vitro activity of danofloxacin were 2 times and 4 times stronger than that of enofloxacin, 8 times and 16 times stronger than that of tylosin, respectively. Following administration danofloxacin intravenously, the concentrations versus time data in healthy and infected chickens (60 each of group) were fitted with a two- compartment model with .?- The main phamacokintic parameters were as following: ta.,. were 0.3400 and 0.5034h; t1128were 7.3 184 and 6.8485h; AUC were 3.2827 and 4.0633mg/1.h: Vd were 16.0631 and 12.1604L/kg; Cl5 were 1.5211 and I .2305L/kg/h.The pharmacokinetics of danofloxacin in plasma and tissues following oral administration5mg/kg were investigated in another two same number groups The concentration-time course in the plasma could be described by two-compartment open model with first 梠rder absorption rate. The concentrations-time course of the liver, kidney and lung were conformed to following triexponential equation; and the muscle performed to bioexponential equation. The main kinetic parameters for the plasma, liver, kidney. heart, 3 lung and muscle in the healthy chickens were as following , respectively: 0. 2428, 0. 3524,0. 3541, 0. 2643, 0. 3377and 0. 2536 (hX t100: 0.8917 ,1.1212,0.7189,l.6142 and 0.4437 (h)0 t1 2~: 8. 7936, 3. 6025, 4. 4570, 10. 2940, 4. 3283 and 5. 9578 (h)0 Tp:0. 9377, 1. 0665, 0. 9362, 0. 9993, 0. 9962 and 1. 4381 (h)0 Cniax :0. 5487 , 2. 8419, 2. 4295, 0. 2668, 1. 7042 and 0. 2726 (~ g/ml) AUC:3.0523, 13.586,10.4180,2.1521,9.6888 and 2.7157(mg/1.h)0 Tcp:12. 613, 18. 0730, 18. 8790, 8. 0557, 19. 693 and 13. 8600~ The main kinetic parameters of the infected chickens for the plasma. liver, kidney. heart. lung and muscle were as following , respectively: 0. 3182, 0. 1743, 0. 2579, 0. 2507, 0. 3335 and 0. 1841 (h)0 t1!20: 1. 5502, 1. 1916, 1.2122,0.8186 and 1. 7968 (h)0 t ~ 12.6200,3.9086,4. 6030, 11. 5326,4. 5960 and 5.9457 (h)~ Tp: 1. 1107,0.8125,0.9286,0.9705. 1.2661 and 1.2211 (h)0 Ctnax: 0. 5106, 2. 7711, 2. 3652,0. 207, 1. 6616 and 0.2367 ( ~g/m1) AUC: 3. 6602, 13. 82, 10. 0830, 1. 8005, 11. 1520 and 2. 2956 (mg/I. h). Tcp: 13. 046, 9. 6900, 17. 5330, 6. 5973, 20. 826 and 12. 408 (hX The pharmacokintic characteristics of danofloxacin in the healthy chickens were as following: it was absorbed rapidly and completely; distributed widely; eliminated slowly; and had excellent bioavailiability following oral administration. The results of tissues kin...