Treatment Of Phenols Wastewater With Biochemistry Method

In this work, industrial phenol wastewaters were treated by biochemistry method, the method can remove these contaminations or mineralized to carbon dioxide, water and various inorganic forms, it is a green technology completely.A new recirculation bioreactor has been proposed and applied to treat industry phenol wastewater. The three phase fluidized bed set, it has the character of highly turbulent flow and rapidly mass transfer. The phenol-degrading consortium was enriched from sludge obtained from Jizhuangzi municipal wastewater plant in Tianjin. The bacteria were isolated two type cells, and have been identified as Klebsiella oxytoca and Brachybacterium rhamnosum by 16SrDNA gene sequence determination. Phenol and 2,4-dichlorophenol biodegradation in recirculation bioreactor were studied using mixture of Klebsiella oxytoca and Brachybacterium rhamnosum. Their degradation processes and the effect factors have been discussed in different technology. The experimental results show that reaction rate using Klebsiella oxytoca and Brachybacterium rhamnosum is faster then Pseudomonas at same temperature or initial phenol concentration. The mathematic model of recirculation bed has been proposed based on the conservative law of mass, heat and momentum transfer as well as the basic equations of thermodynamics,and the numerical solution of the model has been expressed in the text..Here, a new dynamic equation for complex reaction system has been proposed based on statistic mechanics and non-equilibrium thermodynamics , then the new formula has been applied to describe the kinetic behaviors of phenol biodegradation by streptococcus. Experimental results show, the equation can be represent reactive processes pretty well not only in the area between low and high concentration of phenol biodegradation, but also in the range of temperature 25.0℃-32.0℃by using Arrhenius formula. Its confidence level as high as 0.9996, with highly significance. The simulated results from the new formula is much better than Haldane equation in described the reaction processes.Simultaneous quantitative analysis phenol and chlorophenol is needed in study the processes of phenol and chlorophenol biodegradation. Simultaneous determination of multiple component is just focus on chemometrics research. Here, a concept of nonlinear absorbance has been proposed which is correct formula about Beer's law, and then the nonlinear Kalman filter is applied to chemometrics firstly. The binary mixture of phenol and 2-chlorophenol, phenol and 2,4-dichlorophenol, 2-chlorophenol and 4-chlorophenol, 2-chlorophenol and 2,4-dichlorophenol have been analyzed simultaneously based on nonlinear absorbance by UV spectra. Compared the extended Kalman filter with classical least square method, partial least square method and linear Kalman filter , the recovery results showed that the relative derivative about classical least square is biggest, the partial least square and Kalman filter be in same level, derivative of extended Kalman filter is the least one. So the extended Kalman filter method based on nonlinear absorbance is credible and generalized.In this paper, a nonlinear absorbance formula about ternary component mixture has been developed, at the same time, the ternary system about phenol,2-chlorophenol and 2,4-dichlorophenol; as well as 2-chlorophenol, 2,4-dichlorophenol and 2,4,6-trichlorophenol have been analyzed simultaneously. The recovery test showed that the relative derivative of extended Kalman filter is least one in compared with the classical least square method, partial least square method and linear Kalman filter.