| 1. PrefaceThe preface has reviewed the history, the current situation and the future trend of capillary electrophoresis (CE). The fundamental theory of CE and its characters with minimal sample volume requirement, short analysis time and high separation efficiency are introduced simply. Amperometric detection (AD) is applied in this work. The equipment, the principles, the application extension and the developments of CE-AD are summarized in detail. The preface highlights CE application in analyses of active ingredients and fingerprint spectrum of traditional Chinese medicines, and the related active ingredients include alkaloids, flavonoids, phenolic acids, coumarins, glycosides and so on. The recent development of miniaturization of CE is summarized at the last section. 227 references have been referred in this part.2. Determination of Active Ingredients of Polygonum Cuspidatum Sied. et Zucc. by Capillary Electrophoresis with Amperometric DetectionPolygonum cuspidtum Sieb. et Zucc. belongs to the family of polygonaceae, which is a kind of traditional Chinese herbs, and is widely distributed in China. Polygonum cuspidatum Sied. et Zucc. is often used as analgesic, antipyretic, diuretic, expectorant, and antitussive; it is also used for the treatment of arthralgia, jaundice, amenorrhea, chronic bronchitis, and hypertension. trans-Resveratrol, emodin, chrysophanol, and rhein are major important active ingredients in its rhizome and root. High-performance capillary electrophoresis with amperometric detection (CE-AD) was employed to analyze active ingredients of Polygonum cuspidatum Sied. et Zucc. in crude herbs and compound medicines. Operated in a wall-jet configuration, a 300 μm diameter carbon-disk electrode was used as the working electrode, which exhibits good response at +950 mV (vs. SCE) for the four analytes. Under the optimum conditions, these analytes were base-line separated within 27 min in 80 mmol/L boraxbuffer (pH 8.7). Notably, excellent linearity was obtained over two orders of magnitude with detection limit (S/N=3) ranged from 4.2×10-7 to 5×10-8g/mL for all analytes. This method was successfully used in the analysis of Polygonum cuspidatum Sied. et Zucc. and Jindan tablets with relatively simple extraction procedures, and the assay results were satisfactory.3. Determination of Flavonoids in Hippophae rhamnoides L. and its Phytopharmaceuticals by Capillary Electrophoresis with Amperometric DetectionHippophae rhamnoides L. (sea buckthorn) belongs to the Elaeagnaceae family. Intensive research has been undertaken on this ancient plant, revealing that Hippophae rhamnoides L. mainly possesses the therapeutic effects of relieving cough and reducing phlegm, of removing stagnancy of indigested food and relieving flatulence, and of invigorating the circulation of blood and accelerating the stasis assimilation. Approximately 20 flavonoids have been isolated from Hippophae rhamnoides L., and epicatechin, catechin, rutin, kaempferol and quercetin are five most important flavonoids. A high-performance capillary electrophoresis with amperometric detection (CE-AD) method has been developed for the determination of the pharmacologically active flavonoids in Hippophae rhamnoides L. and its extract phytopharmaceuticals in this work. The effects of several factors such as the acidity and concentration of running buffer, the separation voltage, the applied potential and the injection time were investigated to find the optimum conditions. Under the optimum conditions, these analytes were base-line separated within 21 min in 50 mmol/L borax buffer (pH 9.0). Detection limits (S/N=3) ranged from 1.3×10-7 to 5.9×10-7 mg/L for all five analytes. This method was successfully used in the analysis of Hippophae rhamnoides L. and its phytopharmaceuticals after a relatively simple extraction procedure, and the assay results were satisfactory.4. Simultaneous Determination of Phytoestrogens in Different Medicinal Partsof Sophora japonica L. by Capillary Electrophoresis with Amperometric Detecti...
|
PDF Full Text Request