Studies On Resin Mixed Fibrous Adsorber

Membrane chromatography as a competent method has widely used on bioseparation. In order to solve the problem of fouling by impurities, we proposed the new concept of fiber adsorber, which could be used as the medium of chromatography. Another new concept is the fiber chromatography which is loaded with fiber adsorber in some conformations.The mixed functional fiber was chose as research platform. The method of controlled phase transfer was used to prepare the mixed functional fiber adsorber in this work. Resin mixed fibrous adsorbers are a new type of ion exchange adsorbers.To improve the performance of mass transport of these adsorbers a study was carried out to increase the surface porosity and the pore interconnectivity of the adsorber. For this different external liquids were applied in the phase inversion process, such as diethylene glycol monoethyl ether, acetone, tetra-ethylene glycol, and NMP/water mixtures. Using the same external liquid the surface of polyethersulphone matrix was rougher by use of weak cationic resins(CNP80ws) than by use of strong cationic resins(SP112ws). Applying of a high resin loading resulted in a high surface porosity.The resin mixed PES-based fibrous adsorber was developed to study its adsorption and desorption behavior with BSA in this work. The fibrous adsorber with open pore surface had much better adsorption and desorption behavior with higher diffusion coefficient. The adsorption capacity of fibrous adsorber with open pore surface was comparable with the resin's static adsorption capacity. The high loading of resin in fibrous adsorber had high adsorption capacity and high desorption. The adsorption capacity of the adsorber with thin diameter was higher than thick fibrous adsorber. The adsorption isotherm demonstrated that the adsorption was well fitting the langmuir model. As for the mass transport process it could be imaged that the pore diffusion in the pore of fiber matrix and the pore of resin particle should possibly be the rate-determined process.The influence of operation parameters on the behavior of adsorption and desorption of BSA with the weak acidic type cation exchange resin mixed PES-based fibrous adsorber in different structures was also investigated in this work. These factors are pH and flow state of BSA buffer solution, the choice of eluents, pre-treatment and post-treatment of fibrous adsorber , and regeneration of adsorbers etc.In order to reduce non-specific adsorption caused by hydrophobic attraction, Another hydrophilic polymer EVAL was chose as the fiber substrate to form matrix of fiber. Incorporated with resin powder in fiber matrix, the resin CNP80ws mixed EVAL fiber adsorber with some extent of open pore on its surface was prepared by the method of controlled phase transfer. The morphological structures of fiber adsorbers and some factors influenced were investigated. The behavior of adsorption and desorption with model protein BSA was also studied. The results shown that the desorption of resin CNP80ws mixed EVAL fiber adsorber was much better.In this work the hydrophilic polymer used as the fiber adsorber substrate was developed in order to weak the binding of substrate with protein. Poly(vinyl alcohol) was partly modified by acetaldehyde in order to protect the -OH group on the chain of PVA. The modified PVA with aldehyde was easy to get porous UF membrane through phase transfer method. The modified PVA membrane was treated with glutaraldehyde in acidic condition. The aim of this step was to remove aldehyde and crosslink PVA membrane, which was carried out at same time in single step. The result was to get the hydrophilic membrane with good mechanical strength. It could be predicted that this modified PVA hydrophilic porous membrane would be hopefully used as substrate in fiber and/or membrane chromatography.