Preparation And Application Of Molecularly Imprinted Polymer By Attapulgite As Matrix

Molecular Imprinting Technique is originated from bionic to prepare polymers with recognition specificity to template molecules by manual method. Molecular Imprinting Polymers (MIPs) have exhibited extensive application prospect in sensors, catalysis, SPE, chromatography separation and as artificial antibodies in binding assays.With the rapid development of life sciences, materials science and environmental science, and the growing concerns over human health and the environment, analytical science is facing the new opportunity and rigorous challenge. Owing to the diverse and complex of samples, pre-concentration and analysis of trace elements is still necessary before the more aceurate measurement by analytical instruments, which depend on different adsorption materials with higher selectivity and larger adsorption capacity and appropriate separation methods. In this thesis, several new MIPs are synthesized and coupled with high performance liquid chromatography(HPLC) to determine the additive agents in food sample. The more detailed novelty of this research can be categorized as following:1. Using nanoattapulgite as matrix, acrylamide as functional monomer and N, N'-methylene bisacrylamide as crosslinker, both diethylstilbestrol (DES) surface imprinted polymer and non-imprinted polymer were synthesized. The DES imprinted sorbent and non-imprinted sorbent were characterized by FTTR and static adsorption capacity. Compared with each other, the DES imprinted polymer is superior to non-imprinted polymer in adsorption capacity, selectivity and mass transfer property. As the packing material of solid-phase extraction, the DES imprinted polymer has been applied to concentrating DES in pond water and fish samples. A corresponding analytical method to determine diethylstilbestrol has been developed.2. A novel molecularly imprinted polymer was synthesized with attapulgite employed as matrix, sudan I as template molecule,2-vinylpyridine as functional monomer and ethylene glycol dimethacrylate as cross-linking agent, respectively. The imprinted polymer was characterized by the FTIR and TEM. We developed a more simple approach to synthesize sudan I MIP. The vinyl functional group was easily modified onto the surface of ATP with MATMS by silane coupling reaction, and then the modified ATP was directly used as the matrix of MIP, which was time-saving, economic and beneficial to the development of ATP matrix based MIP. Additionally, the MIP-SPE column based on sudan I MIP was prepared and used for the simultaneous on-line SPE to determine sudan I-IV in Yellow River water, tomato sauce and sausage samples.3. Using nanoattapulgite as matrix,β-naphthol as template, methacryloyl-β-cyclodextrins (CyD) as functional monomer and N, N'-methylene bisacrylamide as crosslinker, a novel molecularly imprinted polymer was synthesized by different ultrasonic frequencies and traditional sources of heat. The two kinds of imprinted polymer were characterized by FTTR, TEM and static adsorption capacity. Compared with each other, the DES imprinted polymer prepared by ultrasonic superior to by traditional heat sources in selectivity and mass transfer property to estrone, estradiol, estriol and DES. We chose the MIP prepared at 15 KHz ultrasonic frequencies (SMIP) to simultaneously on-line extract four estrogens in samples from milk. The proposed methods showed the SMIP was feasible in the determination of these estrogens in real samples.4. An imprinted polymer was synthesized using methacrylic acid (MAA) and P-cyclodextrins (CD) as bi-functional monomer for solid-phase extraction of trans-resveratrol (RES).β-CD could self-assembly in the imprinted polymer, which showed that CD could improve the sorbent capacity and the selectivity for RES. Meanwhile, it could avoid to the derivative reaction of CD. The bi-functional monomer imprinted polymers were used as the sorbent for solid-phase extraction to determine RES in wine and Chinese liquor samples. The molecular imprinted polymer solid-phase extraction-high performance liquid chromatography (MIP-SPE-HPLC) method showed higher selectivity and good recoveries higher than 87.9%.