The Study And Application In Cell-Imaging Of Fluorescent Probes For IIB Ions

The activity of life is an effect of different chemical reactions by lots of biological active substances,and metal ion is one important kind of them.Essential trace Zinc participates in many important physiological processes.For example,it plays critical roles in maintaining the structure and realizing the normal function of protein,nucleic acid,peptides,incretion and other big biology molecules.However,Cadmium and mercury are toxic and harmful to organism even at very low concentrations.So the detection of them especially the detection in vivo is important to the research of biology,medicine and environment science.Compared with tranditional methods for the detection of metal ions,the fluorescent probes have attracted much attention over the years for its many inherent merits including high specifity and high sensitivity.This technology has been widely used for the real-time detection of the molecular events both in the chemical and biological systems.We can design and synthesize metal ions fluorescent probes,and study the property of the probes in chemical system,and then detect the concentration changes and distribution of metal ions in cells on line according to confocal fluorescence imaging technology.This will be helpful to explore the function of metal ions in life activity and pathology of the shortness of metal ions and afford essential basis in the chemical and the biological systems.Therefore the BODIPY ratio-fluorescent Cd²⁺ probe BCd1 based on PCT mechanism has been designed and synthesized.The formation of complex BCdl/Cd2²⁺ weakened the electron-donating character,leading to a blue shift of 60 nm in fluorescence emission and an increase of quantum yield for about 4 fold.BCd1 shows strong anti-jamming and low pKa.It can distinguish Cd2²⁺ from Zn²⁺ and especially can be used in ratiometric fluorescence microscopy in Hela cells.The BODIPY fluorescent Hg²⁺ probe BHg1-3 based on PET mechanism have been designed and synthesized with a series of NS₂X₂ catenulate group as recogniation group.The coordination ability and oil/water solubility of the probes can be adjusted by different recognition moiety of catenulate NS₂X₂.The results indicate that comlexation ability between probes and ions is one of most important factors for interference and cell-imaging experiment.The rhodamine derivatives of Hg²⁺ fluorescent probes Rltg1-3 based on Hg²⁺-prompted hydrolysis mechanism have been designed and synthesized.The mechanism was studied and confirmed carefully.RHg1-3 exhibit Hg²⁺ selective among metal ions.RHg series have extremely highly sensitivity for Hg²⁺.RHg1-3 have enabled the detection of ppb level of Hg²⁺ in natural water samples like seawater.More importantly,they hava been also proved of Hg²⁺ fluorescence microscopy in Hela cells.The quinoline derivative of ratio-fluorescent Zn²⁺ probe QZn1 based on mechanism of PCT and deprotonation has been designed and synthesized.The formation of complex QZn1/Zn²⁺ leads to a 90 nm red shift in fluorescence emission,which shows typical ratio changes in spectra.