The Influences Of Arabidopsis Suf3 Mutant In Seed Germination And Embryo Development

In higher plants,embryo development influences the formation of seeds,and seed germination related to the continuation of the specie.Both of them play crucial roles in the development process.Arabidopsis,as a model plant,is an ideal material for studying embryo development and seed germination.Our research shows that Arabidopsis ARP6 play a part in these development processes.Arabidopsis embryo development is a high complicated gene controlled process.There are about 200 genes have been identified which are related to this process.Polarity,cell division, maternal genes and hormones all play important roles in embryo development.Seed germination is also a high complicated process that related to environment cues.In this study,we analyzed the phenotype of loss-of-function ARP6 mutant suf3,and found that it is default in embryo development and seed germination.The suspensors of some suf3 seeds are short and abnormal curvature in 8-cells period.Then we find that there are several devolutive embryos.The germination rate of suf3 seeds is delayed compared with wildtype in the second and third days.GA can partly restore this phenotype,while low concentrations of ABA can increase this phenotype.These results show that GA and ABA pathways both regulate the seed germination phenotype of suf3.NaCl and mannitol stresses also impact the seed germination rate. The suf3 seeds are more sensitive to these stresses than wildtype.Semi-quantitative RT-PCR showed that the expression of the stress-induced gene RD29A in suf3 is lower than in wild type.ARP6 have been found is a component of chromatin modification complex SWR1,which can facilitate the transformation of histone H2A to H2A.Z in order to activate the expression of related genes.Therefore,we speculate that the embryonic development and the seed germination phenotypes of suf3 may be due to abnormal expression of genes in these processes.So many phenotypes of suf3 in different developmental periods indicate that ARP6 is a pleiotropic development regulator which universally regulates the expression of many other genes.