A Systematic Analysis Based On RNase P RNA

In the pre-genomic era, protein research dominates molecular biology, while in post-genomic era, RNA and non-coding DNA sequence studies are leading the theme of the biological research. Evolution theories bias mostly if not exclusively on phylogenetic analysis of proteins, the biased preference for protein analysis caused contradictions in interpreting biological evolution, resulted in the highest gap, neglect of research on RNA's role, and rejection of the biological macroevolution at the molecular level. Particularly the possibility that the intervening noncoding sequences may be transmitting parallel information in the form of RNA molecules may well go down as one of the biggest mistakes in the history of molecular biology. The ubiquitous catalytic ribonuclease P RNA (rnpB) was discovered as a noncoding RNA molecular marker, which conserved highly in all kingdoms of life same as SSU rRNA, including Bacteria, Archaea, and Eucarya, as well as the two major organelles, mitochondria and chloroplasts, responsible for maturation of the 5' end of tRNA and some rRNAs. Scholars think that bacterial and archaeal rnpBs are more similar in sequence and structure than either is to the eukaryal rnpBs by secondary structure comparison; but, eukaryal and archaeal rnpBs display no catalytic activity in vitro without their proteins, and those that are catalytic require extraordinarily high concentrations of both monovalent and divalent salts, distinct from bacteria. All these reports have presented conflicting and confusing results.