| In the present paper, an investigation was carried out on the effects of zinc on ACTH secretion and POMC gene expression in the anterior pituitary in acute cold-exposed rats. So as to further study the function and mechanism of zinc during cold stress.Thirty-two male SD rats were randomized into 4 groups. Rats of Group I and III drank tap water, Group II and Group IV drank high-zinc water (25mg/L), respectively, and 5 days later, rats from Group III and IV underwent exposure to cold at -15℃ for 60 min. All rats were scarified for the detection of plasma ACTH, the level of zinc in the serum and tissues, and the gene expression of proopiomelanocortin (POMC), the precursor for ACTH In the anterior pituitary by dot blot hybridization. Anterior pituitaries of cold-exposed (-15℃, 60min) and unexposed male SD rats (180 to 220g) were dispersed with 0.25%(W/V)trypsin and plated at a density of 1.5 to 1.8×105 cells per well (200μl). The cells were then cultured in an atmosphere of 5% CO2(95% air at 37℃) in DMEM. The medium was changed after 24 hours and different concentrations of Zncl2(10-8 to 10-4mM) were added to the medium and, 12 hours later, the medium was collected for the determination of ACTH.
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