Identification Of A Novel Hypoxia-inducible Gene NDRG2 And Its Primary Founction Studies

NDRG2 (also named SYLD/KIAA1248) , a novel gene that lower expressing in glioma, was first discovered by our laboratory in 1999 with polymerase chain reaction ( PCR )-based subtractive hybridization when looking for the differentially expressed genes between glioma and normal tissue(the GenBank accession No .AF159092)[1], the entire mRNA of NDRG2 is 2024bp in length and it encodes a deduced 357 amino acids unkown protein with a calculated molecular mass of 41 kDa. The NDRG2 gene is located at chromosome 14q11.2, including 16 exons and 15 introns. For the highly homologous in amino acides to N-myc downstream-regulated gene 1(NDRG1), it was named NDRG2 . Ndrg2 was strongly expressed in brain, heart and skeletal muscle ,while weakly expressed or can not detected in other tissues[2]. NDRG2 has been repeatedly isolated by other laboratories and given various names.Although a lot of works had been done on NDRG2 gene in recent years ,its function is still not very clear. In order to discover the true function...