Studies On Sample Labeling Methods Of DNA Microarray And Genome Gene Expression Profiling Of Rubella Virus-infected ECV304 Cells

With the completion of human genome project (HGP), the forefront of the research of modern biology and medicine has moved to structural and functional genomics. DNA microarray, which is one of the most important biochip technologies, has been the major tool for structural and functional genomics studies. The DNA microarray technology has been widely used in the comparative genomics, genome sequencing, SNP analysis, DNA methylation profiling, mRNA expression profiling, gene expression profiling diagnosis, transcription promoter analysis, RNA splicing and microRNA analysis.Virology has been a genomic science for longer than any other field because the first complete genomes sequenced were of viral origin. 1757 full viral genome sequences are available in GenBank. DNA microarray has been applied in the three major parts of structural and functional genomics in virology: (1) virus geneomics study with DNA microarrays including virus strain identification, virus variability analysis and viral transcriptomes. (2) DNA microarray analysis of virally infected host cells. (3) DNA microarray combined with gene knockdown approaches to virogenomics.This thesis includes: (1) Study on sample labeling methods of DNA microarray (2) The cytopathic effects of ECV304 endothelial-like cells induced by rubella...